No 4 (2013)

In normal cell apoptosis and proliferation are strictly controlled by intra- and extracell mechanisms. These signals interfere with cell pathways through cell receptors, estrogen, progesterone, HER2/neu receptors in the case of breast cancer. Expression of apoptotic and proliferative genes is different in breast cancer depending on hormonal status. Besides correlation analysis shows different role of cell receptors in proliferation control in normal and breast tissue.

In model experiments using in vitro virus diarrhea — mucosal disease in cattle (bovine VD-MDC) studied the effectiveness of solvent-detergent (SD) virus inactivation used in the production of intravenous human immunoglobulin G (IgG). Optimized conditions for SD processing of immunoglobulin: the final concentration of tributylphosphate (TBP) 0.3% 0.2% sodium cholate, process temperature of 30 ± 1 °C to 36 ± 1 °C, incubation time 6 hours. The level of reduction of VD-MDC in the processing of the drug in these conditions was more than 6 log 10 TCD 50/ml.

Purpose: To establish the dynamics of tneutrophils ability to aggregation in patients with arterial hypertension (AH) and dyslipidemia receiving rosuvastatin. Examined 30 patients with AH of 1—2 grade and dyslipidemia type IIb, risk of 4, middle age. Control submitted on 26 healthy people of similar age. Patients received rosuvastatin 5 mg per night. In patients with AH and dyslipidemia increased neutrophil aggregation was revealed. As a result the use of rosuvastatin in patients with AH and dyslipidemia during 16 weeks led to normalization of lipids, lipid peroxidation and aggregation of neutrophils.

In this work we have studied the level of C-type natriuretic peptide in patients with respiratory cardiac comorbidities (COPD + HD, COPD + IHD), depending on the genotypes (4a/4b and 4b/4b) of the gene of endothelial nitric oxide synthase. Investigation revealed the influence of VNTR-polymorphism on the level of CNUP under the comorbidity combination of COPD + HD, significantly increased production of CNUP, compared with a single disease. At the same time, in patients with COPD + IHD combination of 4а/4b has no significant effect on CNUP production, which is comparable with a single disease (COPD, IHD).

Internal ureteral stent is widely used in reconstructive urology and radical oncourology. Immune response to stent — a major cause of surgical complications. The development of new materials and coatings will improve bioinert properties of stents and reduce the incidence of complications. Rate X-ray physiology kidney and upper urinary tract, depending on the use of drainage, duration of drainage and timing of passed since the stent was removed in the experiment. X-ray physiology stented kidney was studied in 15 male rabbits breed the “Gray giant” mass of 4350—4580 grams. All animals were fitted both stent. In the left ureter an experimental stent was implanted, in the right as an internal control — polyurethane one of similar diameter. In the pre-and postoperative period X-ray computed tomography by “Philips” tomograph, excretory urography and X-ray cystometria by angiographic complex «Omega Digital Cath» were performed. The use of internal polyurethane stents in the experiment up to a month led to stent obstruction by urinary salts. In the control group noted the frequent development of obstructive uropathy of the stented kidney. Using an experimental stent did not cause violations of the outflow of urine. The original cover of the experimental stent prevents the crystallization of urinary salts on the stent surface. After removal of the polyurethane stent renal dysfunction persisted throughout the observation period. In the main experimental group such complications were not observed.

Carbopol (974 P) was selected as the structure former to make a gel with L-lysine-alpha oxidase. Stability of the gel developed was studied with the use of physico-chemical, rheological and microbiological methods of analysis for the following indicators: the appearance of the gel, odor, pH, uniformity, colloid stability. The authenticity of the activity of L-lysine-a-oxidase in the gel, the viscosity of the gel and microbiological purity of the project comply with manufacturer's monograph L-Lysine-a-oxidase gel, 1% concentration. Studied the specific activity of the developed gel in animal experiments. The results of this study suggest a high wound-healing activity of gel with extract of L-lysine alpha-oxidase of 1%.

The article presents results of clinical case. New pathogen, a gram-negative bacterium Photorhabdus asymbiotica, was isolated. Identified a new causative agent of acute pyelonephritis on the background of urolithiasis — a gram-negative bacterium Photorhabdus asymbiotica, with high sensitivity to modern antibiotics, particularly to fluoroquinolones, eliminating the pathogen for 10 days.