RUDN Journal of Medicine
type: channel
description: EDITOR IN CHIEF: Zhanna D. KOBALAVA, Corresponding Member of the Russian Academy of Sciences, MD, Professor, Head of the Department of Internal Medicine with a course of cardiology and functional diagnostics named after V.S. Moiseev, Medical Institute, RUDN University, Moscow, Russia; ISSN: 2313-0245 (Print) ISSN: 2313-0261 (online) Founded in 1997. Publication frequency: quarterly PUBLISHER: Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University) Open Access: . APC: no article processing charge Peer-Review: double blind. Publication language: Russian, English Indexation: White List, Russian Index of Science Citation, Scopus (Q3 Cite Score), Google Scholar, Ulrich's Periodicals Directory, WorldCat, ResaerchBib, Lens, Research4Life, Dimensions, DOAJ, JournalTOCs, British Library, Bodleian Libraries (University of Oxford), Ghent University Library
publisher: Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)
language: ru-RU
publication name: RUDN Journal of Medicine
issn: 2313-0245
copyright: The right of authorship belongs to the authors of articles. The authors transfer the rights to use the article (including the use and distribution of an article in the Open Access) to the publisher of the journal on a non-exclusive license (Publishing Agreement (Public Offer)). At the same time, authors remain full rightsholders. Publishing Agreement (Public Offer) to Publish an Article in an Academic Periodical 'RUDN Journal of Medicine' The Federal State Autonomous Educational Institution of Higher Education "Peoples’ Friendship University of Russia named after Patrice Lumumba” (RUDN University), represented by the Vice-Rector for Scientific Work Andrey Aleksandrovich Kostin, acting under a Power of Attorney No 0044-09/20-198 dated 29.07.2020, hereinafter referred to as the Publisher, on the one hand, hereby offers to the public at large, hereinafter referred to as the Author, on the other hand, hereinafter collectively referred to as the Parties, to enter into this agreement, hereinafter referred to as the Agreement, regarding publication of scholarly materials, hereinafter referred to as the Article, in the Journal named 'RUDN Journal of Medicine', hereinafter referred to as the Journal, under the following terms. 1. General Terms and Conditions 1.1. Pursuant to Clause 2 of the Article 437 of the Russian Federation Civil Code, this Agreement shall be recognized as the public offer, hereinafter referred to as the Offer. Subject to Article 438 of the Russian Federation Civil Code, this Offer shall be deemed to have been completely and irrevocably accepted after the Author had submitted his/her materials by uploading those onto the network electronic system of the article acquisition for review available at the respective section of the Journal's site at URL: http://journals.rudn.ru/index.php/medicine/author/submit/1 on the Information and Communication Network known as the Internet, hereinafter referred to as the Internet. 1.2. Pursuant to the Russian Federation e…
items: Single cell RNA sequencing: modern approaches and achievements
items: Neuroprotective effect of extracellular vesicles obtained from human glial derivatives on the model of glutamate excitotoxicity
items: Biological and psychological approach to familial hypercholesterolemia
items: A familial case of Marfan syndrome: a novel variant in the FBN1 gene
items: Tumor models in the investigation of oral cancer pathogenesis and treatment development
items: Therapeutic activity of proinflammatory macrophages in endometriosis is driven by their antiproliferative and proapoptotic action
items: Results of a comprehensive drug addiction triptych among Moscow teenagers
items: Oral microbiota importance in the development and prognosis of oropharyngeal region oncological diseases
items: Problems of the dental health in kids from foster families
items: Contribution of Sir Charles Bell to medicine
items: Immunohistochemical study of P53 protein expression in the development of squamous cell carcinoma of the oral mucosa

Single cell RNA sequencing: modern approaches and achievements
type: item
description: Relevance. Single-cell RNA sequencing (scRNA-seq) is a modern approach to studying the diversity and heterogeneity of RNA transcripts in individual cells, as well as to identifying the composition of different cell types and functions in organisms, organs, and tissues. Based on NGS (next-generation sequencing), scRNA-seq provides a vast amount of information at high cellular resolution in various fields, enabling new discoveries in understanding the composition and interaction patterns of individual cell types in humans, animal models, and plants. Despite its rapid development, optimization, and automation worldwide over the past 15 years, scRNA-seq remains relatively new and has only recently been used in Russia. The challenge of mastering and successfully implementing this method is urgent and critical - it is a powerful tool for in-depth analysis and diagnostics, as demonstrated by the results of studies in which it has been used. The aim of the review was to examine the basic principles and steps of scRNA-seq implementation, both in terms of technical implementation and sample preparation as an extension of the classic NGS method, as well as in terms of the complexity and expansion of data processing, and the use of new algorithms and databases. We examined commercially available scRNA-seq technologies and technologies described in scientific literature that have served as prototypes and alternatives. We also examined examples and results of the use of such technologies in various fields of science and medicine, such as oncology, senescence, diagnostics, and clinical trials. Conclusion. Development and successful application of the scRNA-seq method in scientific and clinical practice will become the key to a wide range of future discoveries and successful accurate personalized diagnostics and healthcare.
creator: Artem E. Gusev
creator: Petr V. Chernov
creator: Nikolai A. Dmitriev
creator: Ilya A. Kofiadi
rights: Copyright (c) 2025 Gusev A.E., Chernov P.V., Dmitriev N.A., Kofiadi I.A. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 421
ending page: 435
doi: 10.22363/2313-0245-2025-29-4-421-435

Neuroprotective effect of extracellular vesicles obtained from human glial derivatives on the model of glutamate excitotoxicity
type: item
description: Relevance. Modern research in the field of biomedicine leads to the development of therapeutic drugs based on extracellular vesicles, which are defined as sources of production, as well as targeted modification. In the presented work, for the first time, transcriptome profiling of primary culture of cortical neurons under the influence of extracellular vesicles obtained from glial cells during glutamate excitotoxicity was carried out in order to determine differentially expressed genes. Materials and Methods. Extracellular vesicles were obtained from the conditioned medium of human glial progenitor cells using ultracentrifugation. Model of glutamate excitotoxicity, distributed on the first cultured cortical neurons of cells (P0) with the addition of 100 μM glutamate. Sequencing of prepared libraries of electronic technologies on the NextSeq 1000 platform (Illumina, USA) using the NextSeq 1000/2000 P2 (200 cycles) v3 reagent kit supplemented with 2 % Phix (Illumina) as an internal control. The criterion for statistical innovation of gene expression change between officially recognized FDR< 0.05. Results and Discussion. Transcriptome analysis showed that the addition of extracellular vesicles during glutamate excitotoxicity leads to increased expression of 190 genes and decreased expression of 309 genes (p value < 0.05 and |FC|< 1.5). Gene analysis using the Gene Onthology database showed that genes with increased expression are consistently classified by biological processes. The most represented were: regeneration, reorganization of the extracellular matrix and cytoskeleton, maintenance of homeostasis, activation of the PI3K-Akt pathway and response to cellular stress. Genes with reduced expression were consistently classified into groups: calcium transport, regulation of neuronal processes, apoptosis, glutathergic synapse. These data can indicate that. Extracellular vesicles trigger survival processes in nerve cells when exposed to glutamate and inhibit pathways as…
creator: Margarita O. Shedenkova
creator: Anastasiia A. Gurianova
creator: Anastasia K. Sudina
creator: Egor P. Guguchin
creator: Evgeny A. Karpulevich
creator: Timur Kh. Fatkhudinov
creator: Dmitry V. Goldstein
creator: Diana I. Salikhova
rights: Copyright (c) 2025 Shedenkova M.O., Gurianova A.A., Sudina A.K., Guguchin E.P., Karpulevich E.A., Fatkhudinov T.K., Goldstein D.V., Salikhova D.I. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 436
ending page: 453
doi: 10.22363/2313-0245-2025-29-4-436-453

Biological and psychological approach to familial hypercholesterolemia
type: item
description: Relevance. Familial hypercholesterolemia (FH) is a monogenic hereditary disorder characterized by impaired lipid metabolism. The prevalence of FH in the general population averages 0.32% (95% CI: 0.26-0.39%). The disease can have both autosomal dominant and autosomal recessive inheritance patterns. Eight FH phenotypes associated with mutations in the LDLRAP1, PCSK9, APOA2, APOB, GHR, GSBS, EPHX2, and LDLR genes are known, which can lead to early manifestation of the pathology. The aim of this review is to comprehensively analyze current literature data on the molecular genetics, biological, and psychological aspects of FH. Analysis of signaling pathways in FH revealed three clusters of genes and their encoded proteins responsible for the following processes: assembly, remodeling, and clearance of plasma lipoproteins (genes: LDLR, LDLRAP1, VLDLR, NPC1L1, APOC1, LPA, CETP, MTTP, APOB, PCSK9); cholesterol metabolism (gene: PPP1R17);regulation of plasma lipoprotein particle levels (gene: ANGPTL3). The proteins PCSK9, APOB, and MTTP were identified as key elements (central hubs) of these metabolic networks. The PPP1R17 protein is involved in the mechanisms of long-term depression, a form of synaptic plasticity. Furthermore, the literature describes an association of FH with five other genes: ABCG5, ABCG8, STAP1, CYP7A1, LIPA, and PNPLA5. Conclusion. Thus, for the early diagnosis and effective management of patients with FH, it is necessary to consider not only the expanded spectrum of associated genes and proteins but also the psychological state of patients, particularly their levels of anxiety, depression, and stress.
creator: Leyla V. Tskhovrebova
creator: Anna V. Aghajanyan
creator: Diana D. Bekoeva
creator: Sergey V. Kurevlev
rights: Copyright (c) 2025 Tskhovrebova L.V., Aghajanyan A.V., Bekoeva D.D., Kurevlev S.V. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 454
ending page: 469
doi: 10.22363/2313-0245-2025-29-4-454-469

A familial case of Marfan syndrome: a novel variant in the FBN1 gene
type: item
description: Marfan syndrome is a hereditary connective tissue disorder characterized by marked pleiotropy and clinical variability. The main disease manifestations involve three systems: skeletal, ocular, and cardiovascular. The condition is caused by pathogenic variants in the FBN1 gene, which encodes fibrillin-1, a protein essential for the formation and maintenance of the extracellular matrix. This article describes a familial case (the proband and his father) with clinical manifestations of Marfan syndrome. Whole-genome sequencing of the proband and his father revealed a previously unreported variant, c.5782T>A, p.(Cys1928Ser), in the FBN1 gene. Thus, a molecular genetic diagnosis of Marfan syndrome was established by identifying this novel pathogenic variant. Conclusion. A confirmed diagnosis at both the clinical and molecular genetic levels in both patients determines the further therapeutic strategy and enables timely primary and secondary disease prevention within the family.
creator: Vsevolod I. Stepanenko
creator: Irina Z.H. Zhalsanova
creator: Elizaveta A. Fonova
creator: Daria N. Erburova
creator: Sofia N. Gosudarkina
creator: Ekaterina G. Ravzhaeva
creator: Svetlana V. Fadyushina
creator: Anastasiya A. Nikitina
creator: Gulnara N. Seitova
creator: Olesya I. Klimchuk
creator: Vadim A. Stepanov
creator: Nikolay A. Skryabin
rights: Copyright (c) 2025 Stepanenko V.I., Zhalsanova I.Z., Fonova E.A., Erburova D.N., Gosudarkina S.N., Ravzhaeva E.G., Fadyushina S.V., Nikitina A.A., Seitova G.N., Klimchuk O.I., Stepanov V.A., Skryabin N.A. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 470
ending page: 479
doi: 10.22363/2313-0245-2025-29-4-470-479

Tumor models in the investigation of oral cancer pathogenesis and treatment development
type: item
description: Relevance. Oral cancer is one of the most common cancers among neoplasms of the head and neck. Oral cancer is characterized by a poor prognosis, a lack of specific biomarkers and highly effective targeted treatment. Experimental model systems are needed to study oral cancer pathogenesis and develop new treatments. Understanding the molecular features of oral cancer represents one of the key steps in developing new therapeutic strategies. A wide range of biological models is currently available, but their versatility is limited. Experimental models for studying oral cancer have evolved from cell cultures to in vivo systems that mimic pathological processes and the tumor-stroma interactions. Here, we summarized the available information on the current state of experimental oral cancer systems. In vitro models include immortalized and primary cell lines, spheroids and organoids, whereas in vivo models are represented by syngeneic and xenogeneic models, immunocompromised, immunocompetent, humanized, and genetically engineered animals. In vitro models are effective in studying the biology of oral tumors and evaluating the effectiveness of therapy due to high reproducibility and speed of obtaining results. Existing cell lines are widely used for fundamental and translational research and serve as a crucial component in preclinical trials. In vivo models are used in phase II of preclinical research in drug development and thus represent a transitional stage to clinical trials. Conclusion. Despite significant progress in the development of variousexperimental models, each of them has its own advantages and limitations. There is no universal model that allows for the complete extrapolation of the obtained results to the human body. Therefore, when planning research, it is crucial to select carefully the most suitable biological models based on the objectives at hand.
creator: Maria S. Tretyakova
creator: Elizaveta A. Prostakishina
creator: Elena S. Kolegova
creator: Evgeny L. Choinzonov
creator: Evgeny V. Denisov
rights: Copyright (c) 2025 Tretyakova M.S., Prostakishina E.A., Kolegova E.S., Choinzonov E.L., Denisov E.V. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 480
ending page: 487
doi: 10.22363/2313-0245-2025-29-4-480-487

Therapeutic activity of proinflammatory macrophages in endometriosis is driven by their antiproliferative and proapoptotic action
type: item
description: Relevance. Despite longstanding recognition of endometriosis, there’s an absence of effective treatment. Existing treatments carry significant risk, so research into cell therapy is gaining popularity. Macrophages are a promising agent. A previous study using an animal model demonstrated that introducing proinflammatory macrophages led to a significant reduction in endometriosis lesions. The aim of the study is to analyze the effect of macrophages with a proinflammatory phenotype introduced into an animal model on the proliferation and survival of cells in endometriosis lesions. Materials and Methods. A syngeneic model of endometriosis was obtained in female C57Bl/6 mice by intraperitoneal transplantation of uterine fragments from a donor mouse. RAW264.7 mouse macrophages were utilized as the cellular agent. The animals were then divided into groups: “Control” comprised mice that did not receive macrophage therapy; “Control of therapy” comprised mice that received unpolarized RAW264.7 macrophages; and “Therapy” comprised mice with endometriosis that were injected with RAW264.7 macrophages with a proinflammatory phenotype. Results and Discussion. Consequently, the administration of macrophages with a proinflammatory phenotype resulted in a significant decrease in the production of the proliferation marker protein Ki-67 and a significant increase in the production of effector caspase 3 in the cells of endometriosis lesions compared to the “Control” group. Concurrently, the level of production of the tumor suppressor protein p53, which is involved in the initiation of apoptosis in cells, was comparable to that in the“Control” group. This is in contrast to the group of animals that received unpolarized macrophages. Conclusion. We found that the anti-endometriosis activity of macrophages with a proinflammatory phenotype is associated with the fact that their introduction suppressed the proliferation of endometriosis cells and enhanced their apoptotic death through the ac…
creator: Daria A. Artemova
creator: Andrey V. Elchaninov
creator: Polina A. Vishnyakova
rights: Copyright (c) 2025 Artemova D.A., Elchaninov A.V., Vishnyakova P.A. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 488
ending page: 495
doi: 10.22363/2313-0245-2025-29-4-488-495

Results of a comprehensive drug addiction triptych among Moscow teenagers
type: item
description: Relevance. One of the objectives of the Anti-Drug Strategy of the Russian Federation until 2030, approved by the Decree of the President of the Russian Federation dated November 23, 2020 № 733, is to improve the drug situation monitoring system, increase the efficiency and objectivity of research in the field of drug control. The document also states that the legal regulation of drug trafficking and anti-drug activities should be carried out taking into account the analysis of the drug situation. Along with monitoring drug use, it is extremely important to study the situation with the use of nicotine-containing substances and alcohol (RF Government Resolution of 20.06.2011 № 485). The study of this problem among the teenage population both in Russia and abroad is of particular relevance for the socio-demographic stability of society. The aim. To conduct a comprehensive monitoring of the drug situation among the adolescent population of the city of Moscow. Materials and Methods. The data for Moscow served as the material for the comprehensive study: medical statistics of the narcological service, the results of an anonymous survey of first-year college students, pilot interviewing of experts - specialists of the Moscow drug addiction service, working in the field of prevention of addictive behavior among children and adolescents. Results and Discussion. A comprehensive study was conducted in the period 2016-2023. A trend of decreasing primary appeals of adolescents for drug addiction treatment was revealed, which is primarily due to a reduction in the group of alcohol consumers, despite this, in general, the situation with adolescent abuse of psychoactive substances remains serious. Conclusion. In connection with the obtained results, it is advisable to conduct further comprehensive monitoring of the drug situation among students (teenagers) and develop, on its basis, proposals for preventive measures among children, teenagers and young people, including in the educa…
creator: Aleksey Yu. Abramov
creator: Anton O. Alekseenko
creator: Evgenia A. Koshkina
creator: Dmitry I. Kicha
creator: Victoria V. Arshinova
creator: Lydia V. Shchepanskaya
rights: Copyright (c) 2025 Abramov A.Y., Alekseenko A.O., Koshkina E.A., Kicha D.I., Arshinova V.V., Shchepanskaya L.V. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 496
ending page: 509
doi: 10.22363/2313-0245-2025-29-4-496-509

Oral microbiota importance in the development and prognosis of oropharyngeal region oncological diseases
type: item
description: Relevance. Despite many years of scientific research and clinical results, cancer remains a global health problem. In recent years, there has been an increased interest in studying the role of microorganisms in the occurrence, prognosis and progression of cancer. The probability of isolating carcinogenic microorganisms in the oral microbiota is quite high. In this regard, understanding the role of microorganisms in the occurrence and course of cancer pathology in the future can contribute to the development of innovative strategies for the prevention, treatment of malignant neoplasms and minimization of complications of antitumor treatment. The purpose of this review is a literature analysis of the relationship between representatives of the human oral microbiota in the occurrence and prognosis of cancer. Collection and analysis of 1050 scientific papers using PubMed, Google Scholar and eLIBRARY search engines, published from 2003 to 2024, of which 47 papers are included in this literature review. According to the literature analysis, prevention, diagnostics and treatment of oncological diseases can and should be supplemented by dental approaches aimed at normalizing the microflora. Understanding that oral sanitation significantly reduces its bacterial contamination and, as a result, reduces the number of pathogenic and oncogenic microorganisms can improve the quality of antitumor treatment without significant material and social costs. Conclusion. The use of modern methods of diagnostics and monitoring of oral microbiota in dentistry can become the basis for the development of new strategies for the prevention and treatment of malignant neoplasms of the oral mucosa. Integration of these data into clinical practice requires interdisciplinary cooperation between oncologists, microbiologists and dentists to improve the prognosis of patient treatment.
creator: Anatoliy M. Avanesov
creator: Evgeniya N. Gvozdikova
creator: Yefi Simionidi
creator: Vera A. Titova
rights: Copyright (c) 2025 Avanesov A.M., Gvozdikova E.N., Simionidi Y., Titova V.A. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 510
ending page: 519
doi: 10.22363/2313-0245-2025-29-4-510-519

Problems of the dental health in kids from foster families
type: item
description: Relevance. Dental diseases of the mouth, affecting children of various ages - a nationwide problem of modern society and directly health care. Moreover, the risk of the occurrence and progression of dental diseases among children from socially vulnerable groups is higher, which requires the search for new methods of prevention and treatment. In connection with the increase in the proportion of refused children, the study of the state of health, including dental morbidity, is of particular interest. The aim of the work was to study dental health and the spread of major dental diseases in children in new adoptive families. Materials and Methods. To study dental morbidity in adopted orphans, preventive examinations were carried out in the process of circulation on the basis of dental medical organizations in Kazan in order to identify the main dental diseases, as well as during routine examinations in preschool institutions. The examined children were ranked according to age and gender differences, a total of 35 preschool children took part in the study. Results and Discussion. During the examination of the mouth in children, non-carious dental diseases, cases of manifestations of dentoalveolar pathology, as well as lesions of caries and its complications were revealed. Conclusion. The results obtained on the high prevalence of dental diseases among children from foster families indicate a steady increase in caries and its complications, jugular anomalies, non-carious dental diseases in childrenat special risk due to unfavorable living conditions, aggravated heredity, psychological injuries, nutritional deficiencies, as well as social orphanage, which in general represents a socially significant problem for modern health care.
creator: Madina N. Khadyeva
creator: Afgat N. Galiullin
creator: Svetlana L. Blashkova
rights: Copyright (c) 2025 Khadyeva M.N., Galiullin A.N., Blashkova S.L. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 520
ending page: 531
doi: 10.22363/2313-0245-2025-29-4-520-531

Contribution of Sir Charles Bell to medicine
type: item
description: Relevance. Knowledge of scientific concepts and achievements of the past provides an informative basis for modern research. Moreover, these achievements are filled with the greatest meaning if they are associated with a specific person and the time in which they occurred. Thus, knowledge of the history of science allows us not to neglect the intellectual heritage of past generations and turn it into potential for the development of modern research. In one of his speeches, Nobel laureate Sir Andrew Huxley noted that if he had familiarized himself with the literature of the 19th century in more detail, he would have been able to successfully complete some of his research much faster and more effectively. It must be said that the 19th century gave humanity a whole galaxy of the greatest scientists who made truly fateful discoveries. Our article is dedicated to one of such scientists - Sir Charles Bell (1774-1842), an outstanding Scottish surgeon, anatomist and experimental physiologist. Results of study. In the course of his scientific, teaching and practical medical activities, Bell implemented his accumulated knowledge and experience in the publication of many treatises that remain relevant to this day. Charles Bell applied his talent as an artist in anatomy, personally illustrating magnificent treatises written in collaboration with his older brother, the famous surgeon John Bell, and in surgery (more precisely, in military field surgery), sketching the wounded after the Battle of Waterloo. The scientist also made an invaluable contribution to the development of neurology. Thus, the name of Charles Bell is immortalized in the name of the long thoracic (or «external respiratory») nerve, in the description of lower motor neuron palsy of the facial nerve, and in the «Bell’s sign», when the eyeball rolls upward when trying to close the eye. His description of the differences between the fifth and seventh cranial nerves, as well as the definition of the morpho-functional…
creator: Tatiana A. Fominykh
creator: Sergey A. Kutia
rights: Copyright (c) 2025 Fominykh T.A., Kutia S.A. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 532
ending page: 542
doi: 10.22363/2313-0245-2025-29-4-532-542

Immunohistochemical study of P53 protein expression in the development of squamous cell carcinoma of the oral mucosa
type: item
description: Relevance. Studies on the development of squamous cell carcinoma of the oral mucosa are of paramount importance due to the widespread of the disease and its aggressive course. When histological examination of the development of squamous cell carcinoma of the oral mucosa is not always possible to determine the first signs of malignancy. Squamous cell carcinoma can develop from epithelial hyperplasia and epithelial dysplasia of varying severity. In addition to histological research methods, immunohistochemical method is widely used for tumor diseases, in which the Ki-67 protein is used, with which the proliferation of epithelial cells can be determined, as well as the P53 protein encoding the TP53 gene, which is a suppressor of tumor growth. It is activated only in the presence of damage to the cell genome. The aim was to evaluate the expression of Ki-67 and P53 proteins in the development of dysplasia and squamous cell carcinoma of the oral mucosa. Materials and Methods. Four groups were identified for the study: group 1-16 patients (34.7%) diagnosed with epithelial hyperplasia, group 2-8 patients (17.3%) diagnosed with low-grade epithelial dysplasia, group 3-9 (19.5%) with a diagnosis of “high-grade epithelial dysplasia”, group 4-13 (28.2%) with a diagnosis of “squamous cell carcinoma”. Mouse monoclonal antibodies to Ki-67 (clone MM1, Diagnostic Biosystems, USA) was used to determine cell proliferation. P53 expression was determined using mouse monoclonal antibodies to the P53 protein (Clone D 07, Novocastra, UK). Monoclonal rabbit antibodies P53 (Clone Y5 Epitomics, USA) were used to study only the “mutant type” of the P53 protein. Results and Discussion. The expression of Ki-67 and P53 proteins were observed in all groups. However, the minimum number of immunopositive cells in the study of P53 (Clone D 07) and P53 (Clone Y5) was observed in the epithelial hyperplasia group, and the maximum in the squamous cell carcinoma group. The increase in the number of stained…
creator: Anastasia A. Ivina
creator: Yuliya O. Tigay
creator: Diana Rosina Familia Frias
creator: Olga F. Rabinovich
creator: Igor I. Babichenko
rights: Copyright (c) 2025 Ivina A.A., Tigay Y.O., Familia Frias D.R., Rabinovich O.F., Babichenko I.I. https://creativecommons.org/licenses/by-nc/4.0
license: https://creativecommons.org/licenses/by-nc/4.0
date: 2025-12-18
publication date: 2025-12-18
volume: 29
number: 4
starting page: 543
ending page: 553
doi: 10.22363/2313-0245-2025-29-4-543-553