No 3 (2008)

Methotrexate is one of the most effective and widely used cytostatic drugs. However as all antineoplastic drugs it has very low therapeutic index and high toxicity. Kinetic parameters (rate constants, half-times of restoration and extreme values of concentrations at the beginning of methotrexate infusion) were estimated for biochemical markers It is the method of the quantitative assessment of the drug toxicity. The biokinetic findings allow to set an optimum time interval between infusions of methotrexate for minimization of toxicity at therapeutic efficacy keeping of the antineoplastic drug.

The purpose of this research is the development of approaches to standartization procedure of matrix tincture Bryophyllum e foliis ferm 33b. Particularly, an optimal technique for detecting biologically active substances in Bryophyllum e foliis ferm 33b using thin layer chromatography is obtained. Most parts of pharmacopoeia clause of Russian homeopathic pharmacopoeia for matrix fermented tincture Bryophyllum e foliis ferm 33b are written on the basis of research conducted.

The optimum size of pharmacies network is the basic factor of effective activities. The quantity index of sales units was taken as a measure of its size. The optimum size of drugstore chain was estimated on the basis of the neoinstitutional approach according to which external and internal transaction costs affect the size of pharmacies network. The proposed method makes it possible to correct the structure of the pharmacies network taking into consideration economies scale, advantages and disadvantages of each unique pharmacies network in a particular period of time regarding its strategic targets.

In clause the basic directions of perfection of vocational training and retraining of the pharmaceutical staff in modern conditions are considered and ways of optimization of a professional orientation of training of heads of the pharmaceutical organizations in the field of economy and managements are certain.

Laser correlation spectroscopy (LCS) and analysis of toxicity of patient serum and urine for bovine spermatozoa (AT), new methods for laboratory diagnostics and control of the efficiency of prescribed treatment, were first used in allergological clinical practice. These methods enable screening etiological diagnostics of allergic diseases and evaluation of the severity of the pathological process and state of defense systems of the organism during treatment. Adequate diagnostics makes it possible to realize individual choice of treatment protocol, due to which long-term remission can be expected. We examined 250 patients (mean age 34,4 ± 2 years) with documented allergic disease of medium severity and 145 healthy individuals (mean age 38,1 ± 1 years). Significant differences were revealed in the functional state of the defense systems in these groups. It was found that endogenous intoxication develops in the majority of patients; it is characterized by reduced index of blood toxicity (IBT) and elevated index of urine toxicity (IUT) as well as increased contribution of small particles of blood serum into light scatter. During therapy we observed a decrease in IUT, which attested to correction of the state of humoral defense system of the organism. Alleviation of the pathological state was confirmed by clinical findings. Our results suggest that new methods are highly informative and safe and should be introduced into allergologist's practics.

Develop of apoptosis is a specific program. The sphingomielyne metabolites as ceramide and sphingosine, play an important role in programmed cell death. It is known, that sphingosine is initiate as proliferation and apoptosis. The purpose of this work is studies the roles of sphingosine in apoptosis activation due to acute renal tubular injury. Our experimental data confirm supposition of existence sphingomielyne metabolitic way in activation of apoptosis. We are receive the date with contrary maintenance dynamics sphingomielyne and sphingosine in the kidney cells.

The mouth liquid of 84 patients with healthy periodont was studied and its morphological picture was obtained. The mouth liquid was taken at different time spans from the patients with natural sanitation as well as the sanified ones. It was determined that the types of morphological picture depend on the ratio of organic to mineral components.

Intermediate filament (CK20) and cell cycle regulators (P53, Ki-67) were evaluated by immunohistochemical technique in 32 samples of bladder tumors (Ta - 8, T1-2G1-2 - 20, T1G3 - 3 and T2G3 - 1). Research has shown that intensity of P53 staining allow to defined the grade of tumor cells. Pathological type of CK20 distribution in cells and its high proliferative index (Ki-67) permit to reveal invasive urothelial tumors.

The innovative education system developed by the medical faculty of PFUR for preparation of competitive foreign and domestic doctors on the bases of new forms of continuous post gradual education is presented. The first results of realization of the Innovative educational project, problems and prospects are discussed.