Effect of drought on the antioxidant activity of corn from various soil and climatic regions

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The antioxidant activity of an annual plant Maize Zea of the Armenian population, which grew in various soil-climatic regions of Armenia, was studied. Antioxidant activity by four biochemical parameters was evaluated, the quantitative changes of which under conditions of simulated drought (mild and severe) were determined. Maize Zea of the inbred line B73 was used as the biological control in the experiments. During the experiments, it was found that the Hushakert plant samples have an increased concentration of malonic dialdehyde and flavonoids. In the biological material of corn from Teghut, high concentration values for the restoration of iron and polyphenols by antioxidants are established. The increase in drought from a moderate to the severe biological control of B73 as well as from Armenian maize samples caused the activation of an antioxidant protection system in all four indices was established. This makes it possible to expand the use of maize as a plant indicator, and can be considered as functional foods, as they are a good source of natural antioxidants.

About the authors

Astghik Rafikovna Sukiasyan

National Polytechnic University of Armenia

Author for correspondence.
Email: sukiasyan.astghik@gmail.com

Candidate of Biological Science, Assistant Professor of Faculty of Chemical Technology and Environmental Engineering National Polytechnic University of Armenia

105 Teryan St., 0009, Yerevan, Armenia


  1. Wheeler T, Von Braun J. Climate change impacts on global food security. Science. 2013;341: 508—513. doi: 10.1126/science.1239402
  2. Yoshida T, Mogami J, Yamaguchi-Shinozaki K. ABA-dependent and ABA-independent signaling in response to osmotic stress in plants. Current opinion in plant biology. 2014;21: 133—139. doi: 10.1016/j.pbi.2014.07.009
  3. Pereira A. Plant Abiotic Stress Challenges from the Changing Environment. Frontiers in plant science. 2016;7: 1123. doi: 10.3389/fpls.2016.01123
  4. Rosenzweig C, Elliott J, Deryng D, Ruane AC, Müller C, Arneth A et al. Assessing agricultural risks of climate change in the 21st century in a global gridded crop model intercomparison. Proceedings of the National Academy of Sciences. 2014;111: 3268—3273. doi: 10.1073/ pnas.1222463110
  5. Des Marais DL, Hernandez KM, Juenger TE. Genotype by environment interaction and plasticity: exploring genomic responses of plants to the abiotic environment. The Annual Review of Ecology, Evolution, and Systematics. 2013;44: 5—29. doi: 10.1146/annurev-ecolsys-110512-135806
  6. Todaka D, Shinozaki K, Yamaguchi-Shinozaki K. Recent advances in the dissection of droughtstress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Frontiers in plant science. 2015;6: 84. doi: 10.3389/fpls.2015.00084
  7. Kumar V, Singh A, Mithra SA, Krishnamurthy SL, Parida SK, Jain S et al. Genome-wide association mapping of salinity tolerance in rice (Oryza sativa). DNARes. 2015;22: 133—145. doi: 10.1093/dnares/dsu046
  8. Atteya AM. Alteration of water relations and yield of corn genotypes in response to drought stress. Bulgarian Journal of Plant Physiology. 2003;29: 63—76.
  9. Schnable PS, Ware D, Fulton RS, Stein JC, Wei F, Pasternak S, Liang C. The B73 maize genome: complexity, diversity, and dynamics. Science. 2009;326: 1112—1115.
  10. Sukiasyan AR, Tadevosyan AV, Simonyan GS, Pirumyan GP. The influence of abiotic stress on the growth of plants. Advances in modern natural science. 2016;7: 168—172. (In Russ.)
  11. Hodges D, De Long J, Forney C et al. Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta. 1999;207(4): 604—611. https://doi.org/10.1007/s004250050524
  12. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Anal Biochemistry. 1996;239(1): 70—76.
  13. Gálvez M, Martín-Cordero C, Houghton PJ, Ayuso MJ. Antioxidant activity of methanol extracts obtained from Plantago species. Journal agriculture food chemistry. 2005;53(6): 1927—1933.
  14. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoids content in propolis by two complementary colorimetric methods. Journal of food and drug analysis. 2002;10: 178—182.
  15. Kirakosyan AA, Sukiasyan AR. Ispol’zovaniye yazyka MATLAB v kachestve ekspress-metoda otsenki eksperimental’nykh rezul’tatov [Using MATLAB as an express method for evaluating experimental results]. Informatsionnyye tekhnologii: materialy Mezhdunarodnoy molodezhnoy konferentsii, Yerevan, 23—25 iyunya, 2005 [Information technology: Proceeding of International Conference, Yerevan, 23—25 June 2005]. Yerevan, 2005; pp. 34—37. (In Russ.)
  16. Korosov AV, Gorbach VV. Komp’yuternaya obrabotka biologicheskikh dannykh [Computer processing of biological data]. Petrozavodsk: PetrGU Publ.; 2017. 97 s.
  17. Lobell DB, Banziger M, Magorokosho C, Vivek B. Nonlinear heat effects on African maize a sevidenced by historical yield trials. Nature Climate Change. 2011;1: 42—45. doi: 10.1038/ nclimate1043
  18. Cruzde Carvalho MH. Drought stress and reactive oxygen species: production, scavenging and signaling. Plant Signaling & Behavior. 2008;3: 156—165. doi: 10.4161/psb.3.3.5536
  19. Cornic G. Drought stress inhibits photosynthesis by decreasing stomatal aperture — not by affecting ATP synthesis. Trends plant science. 2000;5: 187—188.
  20. Hoekstra FA, Golovina EA, Buitink J. Mechanisms of plant desiccation tolerance. Trends plant science. 2001;6: 431—438.
  21. Noctor G, Veljovic-Jovanovic S, Driscoll S, Novitskaya L, Foyer CH. Drought and oxidative load in the leaves of C3 plants: a predominant role for photorespiration? Annals of Botany. 2002;89: 841—850.
  22. Smirnoff N. The role of active oxygen in the response of plants to water deficit and desiccation. New Phytologist. 1993;125: 27—58.
  23. Biehler K, Fock H. Evidence for the contribution of the Mehler peroxidase reaction in dissipating excess electrons in drought stressed wheat. Plant physiology. 1996;112: 265—272.
  24. Sukiasyan AR, Tadevosyan AV, Nagdalyan AG, Baghdasaryan SS. Transpiration as a criterion for assessing abiotic stress. Vestnik natsionalnogo politekhnicheskogo universiteta Armenii: gidrologiya i gidrotekhnika. 2015;2: 9—14. (In Russ.)
  25. Sukiasyan AR, Tadevosyan AV, Simonyan GS, Pirumyan GP. The influence of abiotic stress on the growth of plants. Uspekhi sovremennogoy estestvoznaniya. 2016;7: 168—172. (In Russ.)
  26. Sukiasyan AR. Antioxidant capacity of maize corn under drought stress from the different zones of growing. International Journal of Biological, Biomolecular, Agricultural, Food and Biotechnological Engineering. 2016;10(8): 413—416. doi: 10.1999/1307-6892/10005083
  27. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry. 1996;239: 70—76.
  28. Szydlowska-Czerniak A, Dianoczki C, Recseg K et al. Determination of antioxidant capacities of vegetable oils by ferric-ion spectrophotometric methods. Talanta. 2008;76(4): 899—905.
  29. Loo AY, Jain K, Darah I. Antioxidant and radical scavenging activities of the pyroligneous acid from a mangrove plant, Rhizophora apiculata. Food Chemistry. 2007;104(1): 300—307.
  30. Brunetti C, Di Ferdinando M, Fini A, Pollastri S, Tattini M. Flavonoids as antioxidants in plants under abiotic stresses. International Journal of Molecular Sciences. 2013;14(2): 3540—3555.
  31. Schopfer P. Hydrogen peroxide-mediated cell-wall stiffening in vitro in maize coleoptiles. Planta. 1996;199: 43—49. doi: 10.1007/BF00196879
  32. Tsukagoshi H, Busch W, Benfey PN. Transcriptional regulation of ROS control transition from proliferation to differentiation in the root. Cell. 2010;143: 606—616. doi: 10.1016/j.cell.2010.10.020

Copyright (c) 2018 Sukiasyan A.R.

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