Role of Semax and Selank neuropeptides in modulating cell-mediated immunity in the setting of skin burn injury

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Abstract

Relevance. The work describes the results of neuropeptide compounds Semax and Selank in modulating disorders of the cellular link of immunity under experimental burn exposure. The aim — to study the effect of Semax and Selank on the number of white blood cells and phagocytic activity of neutrophils of white rats under experimental burn exposure. Materials and Methods. Burn injury in male rats of 6–8 months of age was modeled by expo animals; animals exposed to burns and not treated burn-exposed animals treated with Semax; animals exposed to burn and treated with Selank. Neuropeptide injections at 100 μg/kg/day were performed intraperitoneally daily for 14 days from thermal burn simulation. To study the immunity parameters, the number of white blood cells and the percentage of lymphocytes, rod and segmented neutrophils were calculated, and the phagocytic activity of neutrophils was assessed. Results and Discussion. It was established that under the conditions of experimental burn exposure, there is an increase in the parameters of the cellular link of immunity: phagocytic activity of neutrophils, phagocytic index, phagocytic number, leukocytic coefficient and number of leukocytes. The activation of granulocyte formation was evidenced by an increase in the rod-nucleating forms of leukocytes (shift of the leukocyte formula to the left). Intraperitoneal injections of the neuropeptide drugs Semax and Selank against the background of thermal skin injury contributed to the correction of the observed changes in white blood parameters and functional neutrophil activity. Conclusion. Thus, under the conditions of a skin burn wound with the use of neuropeptide compounds Semax and Selank, dysfunctional transformations of immunocompetent cells are restored, which confirms the complex effect of Semax and Selank on systemic disorders against the background of local stress, namely, the manifestation.

About the authors

Alfiya K. Azhikova

Astrakhan State Medical University

Author for correspondence.
Email: alfia-imacheva@mail.ru
ORCID iD: 0000-0001-9758-1638
SPIN-code: 1245-3158
Astrakhan, Russian Federation

Marina A. Samotrueva

Astrakhan State Medical University

Email: alfia-imacheva@mail.ru
ORCID iD: 0000-0001-5336-4455
SPIN-code: 5918-1341
Astrakhan, Russian Federation

Lyudmila A. Andreeva

Kurchatov Institute, Institute of Molecular Genetics

Email: alfia-imacheva@mail.ru
ORCID iD: 0000-0002-3927-8590
SPIN-code: 4785-5621
Moscow, Russian Federation

Nikolay F. Myasoedov

Kurchatov Institute, Institute of Molecular Genetics

Email: alfia-imacheva@mail.ru
ORCID iD: 0000-0003-4168-4851
SPIN-code: 1262-2698
Moscow, Russian Federation

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Supplementary files

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1. Fig. 1. Total Number of White Blood Cells of Influence in Background, Burn Injury, and Neuropeptide Treatment with Semax and Selank

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2. Fig. 2. Value of leukocytic coefficient of Influence in Background, Burn Injury, and Neuropeptide Treatment with Semax and Selank

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Copyright (c) 2024 Azhikova A.K., Samotrueva M.A., Andreeva L.A., Myasoedov N.F.

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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