Cytogenetic effect of caffeine in the micronucleus test

Cover Page

Cite item


Relevance. The consumption of caffeine-containing food in the modern world must necessarily be safe for humans, including should not affect the hereditary material of the body. Objective: to determine the possible effect of caffeine at the cytogenetic level by the micronucleus method on erythrocytes. Materials and Methods. The objects for the study were non-linear mice, which were divided into 6 groups - one control group and 5 experimental groups. The first experimental group and the second in the experiment received caffeine in doses of 40 mg/kg and 100 mg/kg.The control group received saline. Caffeine was administered orally. The mutagen (dioxidine) was injected intraperitoneally. On the 5th day of the experimental study, we performed blood sampling for cytogenetic analysis. Results and Discussion. Our study of the caffeine preparation made it possible to determine the following patterns. Firstly, when administered within 5 days, caffeine at a dose of 40 and 100 mg/kg did not cause an increase in the number of micronuclei in erythrocytes in mice. Secondly, the combined use of caffeine (both at a dose of 40mg/kgand at a dose of 100 mg / kg) and dioxidine significantly increased the level of micronuclei in comparison with the control group. Thirdly, caffeine at a dose of 40mg/kgdid not increase the mutagenic activity of dioxidine, but a dose of caffeine of 100mg/kgwhen combined with a mutagen led to a significant increase in the level of cytogenetic damage. Conclusion. According to our data, caffeine in the experimental study was not a mutagen, but at a dose of 100 mg/kg it represented a comutagenic effect.

About the authors

N. A. Durnova

Saratov State Medical University

Author for correspondence.
ORCID iD: 0000-0003-4628-9519
Saratov, Russian Federation

A. R. Klantsataya

Saratov State Medical University

ORCID iD: 0000-0002-5387-1606
Saratov, Russian Federation

M. N. Kurchatova

Saratov State Medical University

ORCID iD: 0000-0003-4432-5555
Saratov, Russian Federation

A. Yu. Karetnikova

Saratov State Medical University

ORCID iD: 0000-0002-8043-3142
Saratov, Russian Federation

A. S. Sheremetyeva

Saratov State Medical University

ORCID iD: 0000-0002-0022-8318
Saratov, Russian Federation


  1. Chu YF. Coffee: emerging health effects and disease prevention. Wiley-Blackwell. 2012. Р. 352.
  2. Porta M, Vioque J, Ayude D, Alguacil J, Jariod M, Ruiz L, et al. Coffee drinking: the rationale for treating it as a potential effect modifier of carcinogenic exposures. European journal of epidemiology. 2003;18(4):289-298.
  3. Kozachuk IV. On the problem of physiological effects of caffeine on human organism. Russian Universities Reports. Mathematics. 2009; 14(1):45-47. (In Russ).
  4. Sivolap YuP, Damulin IV. Сaffeine and Alzheimer’s disease. Neurology Bulletin.2017; 49(4):5-10. (In Russ).
  5. Bohn SK, Ward NC, Hodgson JM, Croft KD. Effects of tea and coffee on cardiovascular disease risk. Food &Function.2012;3(6):575-591.
  6. Proskuryakova TV, Grishin ME. Caffeine and mental health. Psihicheskoe zdorov’e. 2016;14(10):76-82. (In Russ).
  7. Azimova YE, Rachin AP. Migraine, caffeine, ergotamine: the classic trio. Poliklinika. 2016;(1):28-30. (In Russ).
  8. Cano-Marquina A, Tarin JJ, Cano A. The impact of coffee on health. Maturitas. 2013;75(1):7-21.
  9. Zainullin RA, Kunakova RV, Egorova EYu. Coffee, caffeine and human genetics. Beer and beverages. 2015;(6):50-54. (In Russ).
  10. Brambilla G, Martelli A. Update on genotoxicity and carcinogenicity testing of 472 marketed pharmaceuticals. Mutation Research. Reviews in Mutation Research. 2009;681(2-3):209-229.
  11. Golubeva IS, Barmashov AE, Rudakova AA, Baryshnikova MA, Rukk NS, Skryabina AYu, et al.Сytotoxicity of zinc (II) and cadmium (II) iodide complexes with antipyrine, caffeine and phenantroline. Russian Journal of Biotherapy.2017;16(3):75-78. (In Russ). doi: 10.17650/1726-9784-2017-16-3-75-78
  12. Selivyorstov YuA, Babin ME. Caffeine and neurodegenerative disorders. Medical alphabet. 2018; 2 (17(354)):37-42. (In Russ).
  13. Kurpyakova AF, Bykov VN, Chepur SV, Yudin MA, Nikiforov AS. Examination of the effectiveness of a combination of dithionite, ketorolac and caffeine on the model of a rat heavy poisoning by ethanol. Toxicological Review.2011;5(110):14-17. (In Russ).
  14. Kuryleva OM, Gracheva ON, Vyatleva OA, Kuznetsova EG, Salomatina LA, Sevastianov VL. Investigation of specific efficacy of caffeine transdermal therapeutic system on healthy volunteers. Russian Journal of Transplantology and Artificial Organs. 2008;1:40-44.(In Russ).
  15. Severina TG. Effect of caffeine sodium benzoate on the activity of liver lysosomal enzymes and the resistance of rats to acute immersion hypothermia. Voennaj amedicina. 2009;2(51):110-114. (In Russ).
  16. Levikin KE, Kachanov DA, Lapkina GYA, Slobozhanin AA, Pavlysh AV. Comparative effects of antidepressants of various pharmacological groups on the behavior of adult Danio rerio. Reviews on Clinical Pharmacology and Drug Therapy.2020;18(1):51-56. (In Russ). doi: 10.17816/RCF18151-56
  17. Arushanyan EB, Popov AV. Peculiarities of the temporal organization of the behavioral response to caffeine in rats. Experimental and Clinical Pharmacology. 2005;68(1):10-12. (In Russ).
  18. Podolsky IN, Shtrygol SYu, Zubkov VA, Gritsenko IS. Interaction of perspective antidepressant with nootropic properties 2-methyl-3-phenylaminomethylquinolin-4-one with cns stimulants and depressants. Medical Herald of the South of Russia. 2014;(1):80-84. (In Russ). doi: 10.21886/2219-8075-2014-1-80-84
  19. Skamrova GB, Prylutskyy YuI, Evstigneev MP. Combined effect of electromagnetic radiation, dna-intercalators, C60 fullerene and caffeine on human buccal epithelium cells. Biotechnologia Acta. 2014;7(2):54-62. (In Russ).
  20. Durnev AD, Kulakova AV, Zhanataev AK, Oganesyants LA. Evaluation of the cytogenetic and mutagen-modifying activity of caffeine in mouse bone marrow cells. Hygiene and Sanitation. 2015;94(3):106-110. (In Russ).
  21. Ferguson LR, Philpott M. Nutrition and mutagenesis. Annu. Rev. Nutr. 2008;28:313-329.
  22. Mironov AN, Bunyatyan ND, Vasiliev AN, Verstakova OL, Zhuravleva MV, Lepakhin VK, et al. Guidelines for conducting preclinical studies of drugs. Moscow: Neck and K; 2012. (In Russ).
  23. Timson J. Caffeine. Mutation Research. Reviews in Genetic Toxicology. 1977;47:1-52.
  24. Hatzi VI, Karakosta M, Barszczewska K, Karachristou I, Pantelias G, Terzoudi GI. Low concentrations of caffeine induce asymmetric cell division as observed in vitro by means of the CBMN-assay and iFISH. Mutation Research. Genetic Toxicology and Environmental Mutagenesis. 2015;793:71-78.
  25. Woziwodzka A, Gołuński G, Wyrzykowski D, Kaźmierkiewicz R, Piosik J. Caffeine and Other Methylxanthines as Interceptors of Food-Borne Aromatic Mutagens: Inhibition of Trp-P-1 and Trp-P-2 Mutagenic Activity. Chemical Research in Toxicology. 2013;26(11):1660-1673. doi: 10.1021/tx4002513
  26. Durnova NA, Karetnikova AYu., Isaev DS, Klantsataya AR, Sheremetyeva AS. Complex effect of caffeine and dioxidine on behavioral responsesin mice in Porsolt test. RUDN Journal of Medicine. 2020;24(4):315-324. doi: 10.22363/2313-0245-2020-24-4-315-324 (In Russ).
  27. Bunting SY, Lapworth DJ, Crane EJ, Grima-Olmedo J, Koroša A, Kuczyńska A, Mali N, Rosenqvist L, van Vliet ME, Togola A, Lopez B. Emerging organic compounds in European groundwater. Environ Pollut. 2020;3:115945.
  28. Um CY, McCullough ML, Guinter MA, Campbell PT, Jacobs EJ, Gapstur SM. Coffee consumption and risk of colorectal cancer in the Cancer Prevention Study-II Nutrition Cohort. Cancer Epidemiol. 2020;67:101730.

Copyright (c) 2021 Durnova N.A., Klantsataya A.R., Kurchatova M.N., Karetnikova A.Y., Sheremetyeva A.S.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies