REMODELING OF THE TRANSFORMED PHENOTYPE OF CD16+CD32+CD11b+ NEUTROPHILIC GRANULOCYTES OF PATIENTS WITH ATYPICAL CHRONIC BACTERIAL INFECTION IN VITRO

Cover Page

Abstract


The research has shown that the functional state of neutrophilic granulocytes (NG) is determined by the state of NG subpopulation phenotype composition in evaluating the simultaneous expression of CD16, CD32, CD11b membrane markers with considering the density of expressed molecules. The change in NG subpopulations phenotype indicates active or defective inclusion of NG in the immune response in inflammation. Various inducers of endo- and exogenous nature activate NG and promote translocation from cytoplasmic granules and vesicles or expression to the surface cytoplasmic membrane of receptor molecules. In this regard, our interest was in studying the expression peculiarities of CD16, CD32, CD11b NG receptors in patients with atypically occurring bacterial infections (chronic sinusitis) and the possibility of remodeling the NG phenotype under the influence of glucosaminylmuramyl dipeptide (GMDP) and IFNγ in vitro. Subjects of this study were blood samples of patients with chronic sinusitis and conditionally healthy individuals. Flow rate cytometry at CYTOMICS FC500 (Beckman Coulter, USA) was used to evaluate the % NGs expressing CD16, CD32, CD11b, and the fluorescence intensity of these molecules (MFI) before and after incubation with the test substances. Flow cytometry (CYTOMICS FC500, Beckman Coulter, USA) were used to evaluate amount (%) of NGs expressing CD16, CD32, CD11b and mean fluorescence intensity of these molecules (MFI) before and after incubation with the test substances. The studies showed that in patients with chronic sinusitis with persistent recurrent course the presence is characterized by transformed phenotype CD16dimCD32midCD11bbr of CD16+CD32+CD11b+NG subpopulation. We have shown that the revealed changes in the level of expression of trigger membrane receptors do not allow NG to fully engage in inflammatory process and to realize its effector and regulatory functions. Under the influence of GMDP and IFNγ regulatory molecules was demonstrated high mobilization capacity of the studied NGs for functionally significant membrane receptors in realization of various functions.


About the authors

G A Chudilova

Kuban state medical University

Author for correspondence.
Email: chudilova2015@yandex.ru

I V Nesterova

Kuban state medical University; Peoples’ Friendship University of Russia

Email: chudilova2015@yandex.ru

L V Lomtatidze

Kuban state medical University

Email: chudilova2015@yandex.ru

S V Kovaleva

Kuban state medical University

Email: chudilova2015@yandex.ru

T V Rusinova

Kuban state medical University

Email: chudilova2015@yandex.ru

References

  1. Mantovani A., Cassatella M.A., Costantini C., Jaillon S. Neutrophils in the activation and regulation of innate and adaptive immunity. Nat. Rev. Immunol. V. 11. № 8. P. 519—531. doi: 10.1038/nri3024Nat Rev Immunol. 2011 Jul 25.
  2. Silvestre-Roig C., Hidalgo A., Soehnlein O. Neutrophil heterogeneity: implications for homeostasis and pathogenesis. Blood. 2016. V. 127. № 18. P. 2173—2181. doi: 10.1182/blood-2016-01-688887.
  3. Scapini P., Marini O., Tecchio C., Cassatella M.A. Human neutrophils in the saga of cellular heterogeneity: insights and open questions. Immunol Rev. 2016. V. 273. № 1. P. 48—60. doi: 10.1111/imr.12448.
  4. Mandruzzato S., Brandau S., Britten C.M., Bronte V., Damuzzo V., Gouttefangeas C., Maurer D., Ottensmeier C., van der Burg S.H., Welters M.J., Walter S. Toward harmonized phenotyping of human myeloid-derived suppressor cells by flow cytometry: results from an interim study. Cancer Immunol Immunother. 2016. V. 65. № 2. P. 161—169. doi: 10.1007/s00262-015-1782-5.
  5. Beyrau M., Bodkin J.V., Nourshargh S. Neutrophil heterogeneity in health and disease: a revitalized avenue in inflammation and immunity. Open Biol. 2012. V. 2. № 11. P. 120—134. doi: 10.1098/rsob.120134.
  6. Cortjens B., Ingelse S.A., Calis J.C., Valar A.P., Koendetman L., Bem R.A., van Woensel J.B. Neutrophil subset responses in infants with severe viral respiratory infection. Clinical immunology. 2017. V. 176. P. 100—106. doi: 10.1016/j.clim.2016.12.012.
  7. Leliefeld P.H., Wessels C.M., Leenen L.P., Koenderman L., Pillay J. The role of neutrophils in immune dysfunction during severe inflammation. Crit. Care. 2016. V. 20. P. 73. doi: 10.1186/s13054-016-1250-4.
  8. Nesterova I.V., Kolesnikova N.V., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Yevlevsky A.A., Nguyen T.Z.L. A new look at neutrophilic granulocytes: rethinking old dogmas. Infection and immunity. 2017. V. 7. № 3. P. 219—230. doi: 10.15789/2220-7619-2017-3-219-230.
  9. Nesterova I.V., Kolesnikova N.V., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Yevlevsky A.A., Nguyen T.Z.L. A new look at neutrophilic granulocytes: rethinking old dogmas. Infection and immunity. 2018. V. 8. № 1. P. 7—18. doi: 10.15789/2220-7619-2018-1-7-18.
  10. Pillay J., Tak T., Kamp V.M., Koenderman L. Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences. Cell. Mol. Life Sci. 2013. V. 70. № 20. P. 3813—3827. doi: 10.1007/s00018-013-1286-4.
  11. Kobayashi Y. Neutrophil biology: an update. EXCLI J. 2015. V. 14. P. 220—227. doi: 10.17179/excli2015-102. eCollection 2015.
  12. Mare T.A., Treacher D.F., Shankar-Hari M., Beale R., Lewis S.M., Chambers D.J., Brown K.A. The diagnostic and prognostic significance of monitoring blood levels of immature neutrophils in patients with systemic iflammation. Care. 2015. V. 19. P. 57. DOI: 10.1186/ s13054-015-0778-z.
  13. Mócsai A. Diverse novel functions of neutrophils in immunity, inflammation, and beyond. J. Exp. Med. 2013. V. 210. № 7. P. 1283—1299. doi: 10.1084/jem.20122220.
  14. Nesterova I.V., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Kolesnikova N.V., Avdeeva M.G., Rusinova T.V. Differentiation of variants of subpopulations of the transformed cd16+cd11b+ phenotype of neutrophilic granulocytes in acute viral and acute bacterial infections. Immunologija = Immunology. 2016. V. 37. № 4. P. 199—204. doi: 10.18821/0206-4952-2016-37-4-199-204.
  15. Savchenko A.A., Borisov A.G., Kudryavtsev I.V., Gvozdev I.I., Moshev A.V., Cherdantsev D.V., Pervova O.V. Interrelation of the phenotype and metabolism of blood neutrophils in patients with advanced purulent peritonitis in the dynamics of the postoperative period. Infection and Immunity. 2017. V. 7. № 3. P. 259—270. doi: 10.15789/2220-7619-2017-3-259-270.
  16. Garley M., Jabłońska E. Heterogeneity Among Neutrophils. Arch Immunol Ther Exp (Warsz). 2018. V. 66. № 1. P. 21—30. doi: 10.1007/s00005-017-0476-4.
  17. Kruger P., Saffarzadeh M., Weber A.N., Rieber N., Radsak M., von Bernuth H., Benarafa C., Roos D., Skokowa J., Hartl D. Neutrophils: Between host defence, immune modulation, and tissue injury. PLoS Pathog. 2015. V. 11. № 3:e1004651. doi: 10.1371/journal.ppat.1004651.
  18. Nesterova I.V., Evglevskij A.A., Chudilova G.A., Lomtatidze L.V., Kovaleva S.V., Kalashnikov A.E. Features of chromatin restructuring and changes in the level of relative expression of IL8, IL-1β and TNFα genes of neutrophilic granulocytes under the influence of glucosaminylmuramyl dipeptide and interferon-γ in patients with chronic sinusitis in vitro. Immunologija = Immunology. 2015. V. 36. № 6. P. 363—367.
  19. Grayson P.C., Carmona-Rivera C., Xu L., Lim N., Gao Z., Asare A.L., Specks U., Stone J.H., Seo P., Spiera R.F., Langford C.A., Hoffman G.S., Kallenberg C.G., St Clair E.W., Tchao N.K., Ytterberg S.R., Phippard D.J., Merkel P.A., Kaplan M.J., Monach P.A. Neutrophil-related gene expression and low-density granulocytes associated with disease activity and response to treatment in antineutrophil cytoplasmic antibody-associated vasculitis. Arthritis Rheumatol. 2015. V. 67. № 7. P. 1922—1932. doi: 10.1002/art.39153.
  20. Amulic B., Cazalet C., Hayes G.L., Metzler K.D., Zychlinsky A. Neutrophil function: from mechanisms to disease. Annu. Rev. Immunol. 2012. V. 30. P. 459—489. doi: 10.1146/annurev-immunol-020711-074942.

Statistics

Views

Abstract - 306

PDF (Russian) - 212

Cited-By


PlumX

Dimensions


Copyright (c) 2018 Chudilova G.A., Nesterova I.V., Lomtatidze L.V., Kovaleva S.V., Rusinova T.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies