Regulatory cytokine effects in vitro on the phenotype of subpopulations CD62L+CD63-, CD62L+CD63+ and microbicidal activity of neutrophilic granulocytes in patients with colorectal cancer

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Abstract

Relevance. Neutrophilic granulocytes (NG) are the first cells of the immune system to migrate to the tumor and are actively involved in the implementation of a full-fledged antitumor response through the mechanisms of direct killing of tumor cells, extracellular lysis (NET), and through the activation of antibody-dependent cellular cytotoxicity (ADCC), inhibition of angiogenesis, initiation of other cells with antitumor activity. The aim of the study was to study the effect of cytokines IFNα, IFNγ, G-CSF on the CD62L+CD63- and CD62L+CD63+ subsets and the microbicidal activity of NGs in patients with colorectal cancer (CRC) in vitro. Materials and methods. We studied samples of peripheral blood (PB) of 10 patients of both sexes 38-70 years old with newly diagnosed untreated CRC stage II-III (study group) and 10 healthy volunteers (comparison group). The subsets CD62L+CD63+ NG, CD62L+CD63- NG were assessed by flow cytometry (CYTOMICS FC500, Beckman Coulter, USA), the microbicidal functions of NG were tested by cytochemical methods: activity of NADPH - oxidases, myeloperoxidase (MP), level of cationic protein (CP) in spontaneous tests and under additional stress of S. aureus . The effect of IFNα, IFNγ, G-CSF cytokines on subsets and the microbicidal activity of NG in vitro was studied in both study groups. Microsoft Exel 2016 and StatPlus 2010 were used for statistical processing of the obtained data using nonparametric tests: Me (Q1; Q3), Mann-Whitney U-test and Wilcoxon test . Results . The features of transformation of CD62L+ CD63-NG and CD62L+ CD63+ NG subsets of PB in CRC have been established, that allows to get an idea of the NG ability to roll and readiness to activate the microbicidal arsenal, various defects of spontaneous and induced microbicidal activity of oxygendependent and oxygen-independent mechanisms of NG. The effects of cytokine influence on NG in CRC in vitro have been shown, which indicates the possibility of regulating the receptor and microbicidal functions of NG, and, on the other hand, suggests defects in NG perception of regulatory stimuli, that is confirmed by the progression of tumor growth.

About the authors

G. A. Chudilova

Kuban State Medical University

Author for correspondence.
Email: chudilova2015@yandex.ru
SPIN-code: 2092-6412
Krasnodar, Russian Federation

I. V. Nesterova

Kuban State Medical University

Email: chudilova2015@yandex.ru
SPIN-code: 4714-2488
Krasnodar, Russian Federation

S. V. Kovaleva

Kuban State Medical University

Email: chudilova2015@yandex.ru
SPIN-code: 8289-5342
Krasnodar, Russian Federation

L. V. Lomtatidze

Kuban State Medical University

Email: chudilova2015@yandex.ru
SPIN-code: 2060-9316
Krasnodar, Russian Federation

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Copyright (c) 2020 Chudilova G.A., Nesterova I.V., Kovaleva S.V., Lomtatidze L.V.

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