Study and analysis of the resistance of Enterococcus bacteria to antibiotics

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Abstract

Enterococci have acquired resistance to almost all classes of antibiotics. The purpose of this study was to study and analyze the resistance of Enterococcus bacteria to antibiotics isolated from Vladivostok water bodies. The antibiotic resistance of 70 strains of the genus Enterococcus to such antibiotics as tetracycline, streptomycin, erythromycin, ampicillin, levofloxacin, rifampicin, gentamicin, vancomycin was studied by disco diffusion. It turned out that in b. In the b. Zolotoy Rog, more than half of the isolated strains were resistant to streptomycin, erythromycin, and levofloxacin, and in the r. Vtoraya Rechka, more than 50 % of the strains were resistant to streptomycin and rifampicin. The isolated population of enterococcal strains was characterized by multiple resistance to two antibiotics (41 %) in the Second River and three or more antibiotics (45 %) in the Golden River. The most common was resistance to a combination of antibiotics such as streptomycin, rifampicin and erythromycin.

About the authors

Svetlana S. Uskova

Far Eastern Federal University

Email: uskova.ss@yandex.com
ORCID iD: 0000-0001-7515-1207
SPIN-code: 2127-7799

post-graduate student, Institute of the World

Far Eastern Federal University, 10 p. Ajax, island Russian, st. Vladivostok, Primorsky Territory, 690922, Russian Federation

Alina V. Martynova

Far Eastern Federal University

Email: clinmicro@yandex.ru
ORCID iD: 0000-0001-6823-5971
SPIN-code: 1842-1670

Doctor of Medical Sciences, Professor of the Department of Biodiversity and Marine Bioresources, Institute of the World Ocean

Far Eastern Federal University, 10 p. Ajax, island Russian, st. Vladivostok, Primorsky Territory, 690922, Russian Federation

Aleksandra V. Kim

Far Eastern Federal University

Author for correspondence.
Email: kim-sandra@mail.ru
Ph.D. Biol. Sciences, Senior Lecturer, Dept. Biodiversity and Marine Bioresources, Institute of the World Ocean Far Eastern Federal University, 10 p. Ajax, island Russian, st. Vladivostok, Primorsky Territory, 690922, Russian Federation

References

  1. Krasnaya YuV. Significance of bacteria of genus Enterococcus in human activity. Modern problems of science and education. 2014;6:1169–1178. (In Russ.) Available from: https://science-education.ru/ru/article/view?id=16620 https://science-education.ru/ru/article/view?id=16620 (accessed: 17.07.2023).
  2. Veljovic K, Popvic N, Vidojevic M, Tolinacki M, Mihailovic S, Jovcic B, Kojic M. Environmental waters as a source of antibiotic-resistant Enterococcus species in Belgrade, Serbia. Environmental Monitoring and Assessment. 2015;187(9):1–15. http://doi.org/10.1007/s10661-015-4814-x
  3. Hammerum AM. Enterococci of animal origin and their significance for public health. Clinical Microbiology and Infection. 2012;18(7):619–625. http://doi.org/10.1111/j.1469-0691.2012.03829.x
  4. Pashvina DV. Peculiarities of virulence potential of clinical isolates of enterococci gained from amimals: thesis for Candidate of biological science. Orenburg, 2015. 116 p. (In Russ.) Available from: https://www.dissercat.com/content/kharakteristika-virulentnogo-potentsiala-klinicheskikh-izolyatov-enterokokkov-vydelennykh (accessed: 17.07.2023).
  5. Cesare Di A. The marine environment as a reservoir of enterococci carrying resistance and virulence genes strongly associated with clinical strains. Environmental Microbiology Reports. 2014;6(2):184–190. Available: http://doi.org/10.1111/1758-2229.12125
  6. Pasquaroli S. et al. Erythromycin- and copper-resistant Enterococcus hirae from marine sediment and co-transfer of erm(B) and tcrB to human Enterococcus faecalis. Diagnostic Microbiology and Infectious Disease. 2014;80(1):26–28. http://doi.org/10.1016/j.diagmicrobio.2014.06.002
  7. Weiner-Lastinger LM. et al. Antimicrobial-resistant pathogens associated with adult healthcare-associated infections: summary of data reported to the National Healthcare Safety Network, 2015–2017. Infection Control Hospital Epidemiology. 2020;41:1–18. http://doi.org/10.1017/ice.2019.296
  8. Korajkic A, McMinn BR, Staley ZR, Ahmed W, Harwood VJ. Antibiotic-resistant Enterococcus species in marine habitats: A review. Current Opinion in Environmental Science & Health. 2016;16:92–100. http://doi.org/10.1016/j.coesh.2020.07.003
  9. Hicks LA, Bartoces MG, Roberts RM, Suda KJ, Hunkler RJ, Taylor TH, Schrag SJ. US outpatient antibiotic prescribing variation according to geography, patient population, and provider specialty in 2011. Clinical Infectious Diseases. 2015;60:1308–1316. http://doi.org/10.1093/cid/civ076
  10. Fleming-Dutra KE. et al. Prevalence of inappropriate antibiotic prescriptions among US ambulatory care visits, 2010-2011. Journal of American Medical Association. 2016;315:1864–1873. http://doi.org/10.1001/jama.2016.4151
  11. Zemlyanko OM. Mechanisms of multiresistance of bacteria to antibiotics. Ecological genetics. 2018;16(3):4–17. (In Russ.) URL: https://cyberleninka.ru/article/n/mehanizmy-mnozhestvennoy-ustoychivosti-bakteriy-k-antibiotikam (accessed: 12.07.2023).
  12. Egorov NS. Basics of study of antibiotics./textbook 6 ed. rewised and added. Moscow: Moscow State University, Science. 2004. 580 p. (In Russ.) Available from: http://www.vixri.ru/d3/Egorov%20%20_Osnovy%20uchenija%20ob%20antibiotikax,%20izd.6,%202004,%20528s.pdf (accessed: 16.07.2023).
  13. Acar JF, Buu-Hoi AY. Resistance patterns of important Gram-positive pathogens. Journal of Antimicrobial Chemotherapy. 1988;21:41–47. http://doi.org/10.1093/jac/21.suppl_c.41
  14. Nakayama J, Ruhfel RE, Dunny GM, Isogal A, Suzuki A. The prgQ gene of the Enterococcus faecalis tetracycline resistance plasmid pCF10 encodesa peptide inhibitor, iCF10. Journal of Bacteriology. 1994;176(23):7405–7408. http://doi.org/10.1128/jb.176.23.7405-7408.1994
  15. Huys G, D’Haene K, Collard J, Swings J. Prevalence and Molecular Characterization of Tetracycline Resistance in Enterococcus Isolates from Food. Applied and Environmental Microbiology. 2004;70(4):1555–1562. http://doi.org/10.1128/AEM.70.3.1555-1562.2004
  16. Chow JW. Aminoglycoside Resistance in Enterococci. Clinical Infectious Diseases. 2000;31(2):586–589. http://doi.org/10.1086/313949
  17. Leclercq R. et al. Resistance of enterococci to aminoglycosides and glycopeptides. Clinical Infectious Diseases. 1992;15(3):495–501. http://doi.org/10.1093/clind/15.3.495
  18. Zou LK. et al. Erythromycin resistance and virulence genes in Enterococcus faecalis from swine in China. New Microbiologica. 2011;34(1):73–80. Available from: https://www.newmicrobiologica.org/PUB/allegati_pdf/2011/1/73.pdf (accessed 17.07.2023).
  19. Rafailidis PI, Ioannidou EN, Falagas ME. Ampicillin/Sulbactam in Severe Bacterial Infections. Review Article. 2007;67(13):1829–1849. http://doi.org/10.2165/00003495-200767130-00003
  20. Rice LB. Beta-lactam antibiotics and gastrointestinal colonization with vancomycin-resistant enterococci. The Journal of Infectious Diseases. 2005;24(12):804-814. http://doi.org/10.1007/s10096-005-0057-z
  21. Marshall SH, Donskey CJ, Hutton-Thomas R, Salata RA, Rice LB. Gene dosage and linezolid resistance in Enterococcus faecium and Enterococcus faecalis. Antimicrobial Agents and Chemotherapy. 2002;46(10):3334–3336. http://doi.org/10.1128/AAC.46.10.3334-3336.2002
  22. Oyamada Y, Ito H, Fujimoto K, Asada R. et al. Combination of known and unknown mechanisms confers high-level resistance to fluoroquinolones in Enterococcus faecium. Journal of Medical Microbiology. 2006;55(6):729–736. http://doi.org/10.1099/jmm.0.46303-0
  23. Sedova MK. Development of content and methods of quality control of hard drug substance of levofloxacine: thesis of candidate pharmacological science. Moscow; 2016. 167 p. (In Russ.) Available from: http://www.dslib.net/farmakognozia/razrabotka-sostava-i-metodov-kontrolja-kachestva-tverdoj-lekarstvennoj-formy.html (accessed: 17.07.2023).
  24. Yasufuku T. et al. Mechanisms of and Risk Factors for Fluoroquinolone Resistance in Clinical Enterococcus faecalis Isolates from Patients with Urinary Tract Infections. Journal of Clinical Microbiology. 2011;49(11):3912–3916. http://doi.org/10.1128/JCM.05549-11
  25. Rattanaumpawan P, Tolomeo P, Bilker WB, Fishman NO, Lautenbach E. Risk factors for fluoroquinolone resistance in Enterococcus urinary tract infections in hospitalized patients. Epidemiology and Infections. 2011;139:955–961. http://doi.org/10.1017/S095026881000186X
  26. Enne VI, Delsol AA, Roe JM, Bennett PM. Rifampicin resistance and its fitness cost in Enterococcus faecium. Journal of Antimicrobial Chemotherapy. 2004;53(2):203–207. http://doi.org/10.1093/jac/dkh044
  27. Aslangul E, Massias L, Meulemans A, Chau F, Andremont A, Courvalin P, Fantin B, Ruimy R. Acquired Gentamicin Resistance by Permeability Impairment in Enterococcus faecalis. Antimicrobial Agents and Chemotherapy. 2006;50(11):3615–3621. http://doi.org/10.1128/AAC.00390-06
  28. Jolivet S. et al. First nosocomial outbreak of vanA-type vancomycin-resistant Enterococcus raffinosus in France. Journal of Hospital Infection. 2016;94 (4):346–350. http://doi.org/10.1016/j.jhin.2016.09.004
  29. Cetinkaya Y, Fallk P, Mayhall C. Vancomycin-Resistant Enterococci. Clinical Microbiology Review. 2000;13(4):686–707. http://doi.org/10.1128/CMR.13.4.686
  30. Hijazi NM, Elmanama AA, Al-Hindi A. Vancomycin-Resistant Enterococci in Fecal Samples from Hospitalized Patients and Non-Hospitalized Individuals in Gaza City. Journal of Public Health. 2009;17(19):243–249. http://doi.org/10.1007/s10389-008-0242-5

Copyright (c) 2023 Uskova S.S., Martynova A.V., Kim A.V.

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