Stabilization of petroleum and oil emulsions by bioorganic compositions based on humic acids

Cover Page

Cite item

Full Text

Abstract

The stabilizing ability of humic acids isolated from reed fen peat of the Ryazan region in the relation to oil and petroleum products under various abiotic conditions of the aquatic environment has been studied. The applicability of the used biological products in conditions of low temperature and increased salinity of the medium is determined. The stabilizing ability of the developed biological products was determined by the transmittance of emulsions. It was found that in the condition of increased salinity, the transmission coefficient decreases by 5-24% relative to the control experiment for crude oil, diesel fuel and waste oil when using monocultural biological products. It was found that the use of polycultures in the composition of biological products significantly reduces the transmittance by 24-43% for “HAs from reed peat of the Ryazan region + Rhodococcus erythropolis S67 + Rhodococcus erythropolis X5” and 10-29% for “Humic acids of reed peat of the Ryazan region + Rhodococcus erythropolis S67 + Rhodococcus erythropolis X5 + Pseudomonas fluorescens 142NF”. The applicability of the studied biological products in conditions of low temperatures is confirmed by a decrease in the transmission coefficient to 68-73% for diesel fuel, to 60-64% for used synthetic engine oil and to 64-69% for crude oil. It was found that the transmission coefficient decreases in the series: “Humic acids of reed fen peat of the Ryazan region + Rhodococcus erythropolis X5” → “Humic acids of reed peat of the Ryazan region + Pseudomonas fluorescens 142NF” → “Humic acids from reed fen peat of the Ryazan region + Rhodococcus erythropolis S67”. It was revealed that the reduction of the transmission coefficient of oil emulsions occurs due to the combined use of humic acids isolated from reed peat of the Ryazan region and the association of bacteria (both when using 2 and 3 strains). The stabilizing ability of humic acids in conditions of increased salinity was comparable to it in conditions of reduced temperature. It has been established that cane humic acids of the Ryazan region can be used as a basis for biological products that stabilize oil and oil emulsions.

About the authors

Maria M. Gertsen

Tula State Lev Tolstoy Pedagogical University

Author for correspondence.
Email: mani.gertsen@gmail.com
ORCID iD: 0000-0002-0026-4933
SPIN-code: 3989-8560

Junior Researcher at the Laboratory of Chemistry and Soil Ecology, Assistant at the Department of Biomedical Disciplines and Pharmacognosy

125 Lenin Avenue, Tula, 300026, Russian Federation

Anastasia N. Golysheva

Tula State University

Email: nastyagolysheva2000@yandex.ru
ORCID iD: 0009-0004-5135-4455
SPIN-code: 1852-6831

Junior Researcher at the Laboratory of Chemical Conversion of Renewable Biomass and Organic Synthesis

92 Lenin Avenue, Tula, 300012, Russian Federation

Leonid V. Perelomov

Tula State Lev Tolstoy Pedagogical University

Email: perelomov@rambler.ru
ORCID iD: 0000-0003-0507-4968
SPIN-code: 2695-0200

PhD in Biology, Senior Researcher at the Laboratory of Chemistry and Ecology of Soils, Associate Professor, Department of Chemistry

125 Lenin Avenue, Tula, 300026, Russian Federation

References

  1. Ivanenko NV. Ecological toxicology. Study guide. Vladivostok: Publishing house of VSUES$ 2006. 108 p. (In Russ.)
  2. Neustroev MM. Ecological assessment of oil-contaminated permafrost soils and the development of methods for their bioremediation (abstract of the dissertation). Yakutsk; 2016. 16 p. (In Russ.)
  3. Grechishcheva NYu. Development of scientific bases of application of humic substances for elimination of consequences of oil pollution of soil and water environments (diss. of candidate of chemical sciences). Moscow: MSU; 2016. 326 р. (In Russ.)
  4. Herzen MM, Dmitrieva ED. The ability of humic acids to stabilize emulsions of oil and petroleum products; Vestnik TvGU. Chemistry series. 2020;3(41):103–111. http://doi.org/10.26456/vtchem2020.3.11 (In Russ.)
  5. Dmitrieva ED., Syundyukova KV., Leontieva MM., Glebov NN. Influence of the pH of the medium on the binding of heavy metal ions by humic substances and himatomelanic acids of peat. Scientific notes of Kazan University. Natural sciences. 2017;159(4):575–588. (In Russ.)
  6. Gostischeva MV, Fedko IV, Pisnichenko EO. Comparative characteristics of methods for extracting humic acids from peat in order to obtain humic preparations. Reports of Tomsk State University of Control Systems and Radioelectronics. 2004;1(9):66–68. (In Russ.)
  7. Izosimov AA. Physico-chemical properties, biological activity and detoxifying ability of humic preparations differing in the genesis of organic raw materials. Moscow; 2016. (In Russ.)
  8. Maltseva EV, Yudina NV. Sorption of humic acids by quartz sands. Solid fuel chemistry. 2014;4:27–27. http://doi.org/10.7868/S0023117714040082 (In Russ.)
  9. Bakeeva RF, Karimullin RA, Kurabasheva RF, Gorbunova TS, Vakhitova OE, Sopin, VF. Micelle formation in a binary dimethyl sulfoxide-water solvent in the presence of NaCl. Planning and optimization of the experiment. Liquid crystals and their practical use. – 2017;17(2):51–61. http://doi.org/10.18083/LCAppl.2017.2.51 (In Russ.)
  10. Grechishcheva NYu, Han P, Budylin SV, Permnova IV, Meshcheryakov SV. Investigation of the binding ability of modified humic preparations with respect to pyrene in homogeneous and heterogeneous phases. Environmental protection in the oil and gas complex. 2011;6:24–29 (In Russ.)
  11. Farmakovskaya TA, Novozhilova TI, Makartsev VV, Karasev AV, Konstantinova OV. Development of methods for determining the stability of fluorocarbon emulsions and forecasting their shelf life. Chemistry and technology of organic substances. 2017;1:90–99. http://doi.org/10.54468/25876724_2017_1_90
  12. Boikova OI, Volkova EM. Chemical and biological properties of peat of the Tula region. News of TulSU. Natural sciences. 2013;3:253–264. (In Russ.)
  13. Herzen MM, Dmitrieva ED. The ability of humic acids of peat to stabilize emulsions of oil and petroleum products. Bulletin of Tver State University. Series: Chemistry. 2020;3:103–111. http://doi.org/10.26456/vtchem2020.3.11 (In Russ.)
  14. Herzen MM, Dmitrieva ED. Stabilizing ability of humic substances and microorganisms of the genus Rhodococcus in relation to petroleum products. TvSU Bulletin. Chemistry series. 2020;3(41):112–123. http://doi.org/10.26456/vtchem2020.3.12 (In Russ.)
  15. Grechishcheva NYu, Shchukina VD, Kholodov VA, Lazareva EV, Parfenova AM, Meshcheryakov SV, Perminova IV. Assessment of the ability of humic-clay complexes to stabilize oil emulsions in water. Environmental protection in the oil and gas complex. 2014;9:51–55. (In Russ.)
  16. Nechaeva IA, Lyong TM, Satina VE, FilonovAE, Ponamoreva ON. The effect of temperature on the ability of rhodococci – effective petrodestructors to absorb hexadecane. Actual biotechnology. 2016;3(18):103–106. (In Russ.)
  17. Yudina NV, Savelyeva AV, Lomovsky OI. Surface-active properties and biological activity of mechanoactivated humic acids isolated from peat. Chemistryin the interests of sustainable development. 2019;27(4):437–442. http://doi.org/10.15372/KhUR2019156 (In Russ.)
  18. Mozgovaya ND. Investigation of the processes of association formation and surfaceactive properties of humic acids depending on the concentration and pH of the medium: thesis (project) of a specialist in the field of training. 04.05. 01 – Fundamental andapplied chemistry. 2018. (In Russ.)
  19. Ji S, Jikuiang D, Pyu D, Ye L, Xiashuang C, Haili H. Preparation and evaluation of properties of stable foams obtained on the basis of heavy oil. Petrochemistry. 2017;57(2):226–234. http://doi.org/10.7868/S0028242117020071 (In Russ.)
  20. Rahman KSM, Banat IM, Thahira J, Thayumanavan T, Lakshmanaperumalsamy P. Bioremediation of gasoline contaminated soil by a bacterial consortium amended with poultry litter, coir pith and rhamnolipidbiosurfactant 300. K.S. Rahman, I.M. Banat, J. Tharira, T. Thauyumanavan, P. Lakshmanaperumalsamy. Bioresource Technology. 2002;81:25–32. http://doi.org/10.1016/S0960-8524(01)00105-5
  21. Panov AV. Changing the composition of communities of destructive bacteria in conditions of contamination with stable organic compounds (abstract of the dissertation). Pushchino; 2013. 24 р. (In Russ.)
  22. Nechaeva IA, Luong TM., Satina VE, Filonov AE, Ponamoreva ON. Influence temperatures on the ability of rhodococci – effective petrodestructors to absorb hexadecane. Actual biotechnology. 2016;3(18):103–106. (In Russ.)
  23. Chaika NYa, Rejepova AA, Akhmetov LI, Puntus IF, Petrikov KV, Filonov AE. Features of growth and production of bio-surfactants of the psychrotrophic strain-oil destructor Rhodococcus erythropolis F2-2 when cultivated on different substrates at low temperature. Mechanisms of adaptation of microorganisms to various environmental conditions. 2022. p. 284–286. (In Russ.)

Copyright (c) 2023 Gertsen M.M., Golysheva A.N., Perelomov L.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies