Rat hematological parameters of subacute multifrequency electromagnetic next-generation cellular communications exposure

Cover Page

Cite item


The cellular communication electromagnetic fields have become a significant physical environmental factor recently, which cannot but cause reasonable concern due to the possible adverse effects of human health. Experimental laboratory animal studies are an integral part of the development and substantiation maximal permissible levels, in which the blood system is an objective functional indicator of the electromagnetic field biological effects. Thus, the purpose of the research was to study the hematological parameters of the male and female rat blood of subacute multi-frequency electromagnetic exposure from cellular communication systems and possible long-term biological effects. The object of research was male and female Wistar line rats. There were multi-frequency 30-days electromagnetic field exposure with 500 μW/cm2 power density at 3.5, 28 and 37 GHz summary. Hematological parameters of peripheral rat blood of the experimental and control groups were determined at the end of 30 days of exposure and 30 days after exposure. 30 days exposure did not lead to statistically significant changes in the blood cell composition in either male or female rats compared with sham exposure. In post exposure period (30 days after), significant changes in hematological parameters shown female rats only, which consisted in a decrease in the average content (MCH) and the average concentration (MCHC) of hemoglobin (Hb) in erythrocyte (RBC), the level of leukocytes (WBC), lymphocytes (LYM) and an increase in monocytes (MON). There were no statistical changes in all hematological parameters in male rats. The research results suggest the development of aftereffect adaptive reactions in rats upon completion of subacute electromagnetic exposure and their correlation to the effect depending on the sex of the animal.

About the authors

Valentina S. Orlova

Peoples’ Friendship University of Russia (RUDN University)

Email: bte2005@mail.ru
SPIN-code: 8078-0470
Doctor of Biological Sciences, Professor of the Department of Environmental Security and Product Quality Management, Institute of Environmental Engineering 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation

Sergey Yu. Perov

Izmerov Research Institute of Occupational Health

Email: perov@irioh.ru
ORCID iD: 0000-0002-6903-4327

Doctor of Biological Sciences, Head of the Laboratory of Electromagnetic Fields

31 Budyonnogo Prospekt, Moscow, 105275, Russian Federation

Rano Z. Lifanova

Peoples’ Friendship University of Russia (RUDN University); Izmerov Research Institute of Occupational Health

Author for correspondence.
Email: torazo-414@mail.ru
ORCID iD: 0000-0002-8747-9856

postgraduate student, Department of Environmental Security and Product Quality Management, Institute of Environmental Engineering, Peoples’ Friendship University of Russia (RUDN University); research fellow, Izmerov Research Institute of Occupational Health

6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation; 31 Budyonnogo Prospekt, Moscow, 105275, Russian Federation

Sergei A. Pinegin

State Scientific Center of the Russian Federation Institute of Biomedical Problems, Russian Academy of Sciences

Email: serg.pineqin@gmail.com
postgraduate student, researcher fellow 76A Khoroshevskoe Shosse, bldg 34, Moscow, 123007, Russian Federation


  1. Gajšek P, Ravazzani P, Wiart J, Grellier J, Samaras T, Thuróczy G. Electromagnetic field exposure assessment in Europe radiofrequency fields (10 MHz - 6 GHz). J. Expo. Sci. Environ. Epidemiol. 2015;25(1):37-44. https://doi.org/10.1038/jes.2013.40
  2. Chiaramello E, Bonato M, Fiocchi S, Tognola G, Parazzini M, Ravazzani P, Wiart J. Radio frequency electromagnetic fields exposure assessment in indoor environments: a review. Int. J. Environ. Res. Public Health. 2019;16(6):955. https://doi.org/10.3390/ijerph16060955
  3. Sienkiewicz Z, Calderón C, Broom KA, Addison D, Gavard A, Lundberg L, Maslanyj M. Are exposures to multiple frequencies the key to future radiofrequency research? Front. Public Health. 2017;5(328):11. https://doi.org/10.3389/fpubh.2017.00328
  4. Zhu R, Wang H, Xu X, Zhao L, Zhang J, Dong J, Yao B, Wang H, Zhou H, Gao Y, Peng, R. Effects of 1.5 and 4.3 GHz microwave radiation on cognitive function and hippocampal tissue structure in Wistar rats. Sci. Rep. 2021;11(1):1-12. https://doi.org/10.1038/s41598-021-89348-4
  5. Shirai T, Wang J, Kawabe M, Wake K, Watanabe SI, Takahashi S, Fujiwara O. No adverse effects detected for simultaneous whole-body exposure to multiple-frequency radiofrequency electromagnetic fields for rats in the intrauterine and pre-and post-weaning periods. J. Radiat. Res. 2017;58(1):48-58. https://doi.org/10.1093/jrr/rrw085
  6. Darvishi M, Mashati P, Kandala S, Paridar M, Takhviji V, Ebrahimi H, Zibara A, Khosravi A. Electromagnetic radiation: a new charming actor in hematopoiesis? Expert Rev. Hematol. 2021;14(1):47-58. https://doi.org/10.1080/17474086.2020.1826301
  7. El-Bediwi AB, Saad M, Elkott AF, Eid E. Influence of electromagnetic radiation produced by mobile phone on some biophysical blood properties in rats. Cell Biochem. Biophys. 2012;65(3):297-300. https://doi.org/10.1007/s12013-012-9432-4
  8. Alghamdi MS, El-Ghazaly NA. Effects of exposure to electromagnetic field on of some hematological parameters in mice. Open Journal of Medicinal Chemistry. 2012;2(2):30-42. http://doi.org/10.4236/ojmc.2012.22005
  9. Usman AD, Ahmad WW, Ab Kadir MZ, Mokhtar M, Ariffin R. Effect of radiofrequency electromagnetic field exposure on hematological parameters of mice. World Appl. Sci. J. 2012;16(5):656-664.
  10. Yashchenko SG, Rybalko SYu, Karpovich AV. Hematological parameters of human peripheral blood under the influence of electromagnetic radiation of mobile phones. Ukrainian Medical Almanac. 2014;17(3):109-111. (In Ukr.)

Copyright (c) 2022 Orlova V.S., Perov S.Y., Lifanova R.Z., Pinegin S.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies