Evaluation of effectiveness of solvent-detergent viral in activation in the manufacture of intravenous human immunoglobulin G in a model of virus diarrhea — mucosal disease of cattle

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  • Authors: Kaz’yanin A.V., Zubkova N.V., Krasilnikov I.V., Lobastova A.K., Anastasiev V.V., Nikolaeva A.M., Glotova T.I., Kungurtseva O.V., Glotov A.G.
  • Issue: No 4 (2013)
  • Pages: 24-30
  • Section: Articles
  • URL: http://journals.rudn.ru/medicine/article/view/3357

Abstract


In model experiments using in vitro virus diarrhea — mucosal disease in cattle (bovine VD-MDC) studied the effectiveness of solvent-detergent (SD) virus inactivation used in the production of intravenous human immunoglobulin G (IgG). Optimized conditions for SD processing of immunoglobulin: the final concentration of tributylphosphate (TBP) 0.3% 0.2% sodium cholate, process temperature of 30 ± 1 °C to 36 ± 1 °C, incubation time 6 hours. The level of reduction of VD-MDC in the processing of the drug in these conditions was more than 6 log 10 TCD 50/ml.

A V Kaz’yanin

Federal budgetary institute “Perm State Pharmaceutical Academy”

Email: a.kazyanin@mail.ru

N V Zubkova

FSUC “SIC Microgen” of MH RF Nizhniy-Novgorod Affiliate “ImBio”

Email: zubkova@imbio.ru

I V Krasilnikov

FSUC “SIC Microgen” of MH RF

Email: kiv06@mail.ru

A K Lobastova

FSUC “SIC Microgen” of MH RF

Email: a.k.lobastova@microgen.ru

V V Anastasiev

FSUC “SIC Microgen” of MH RF Nizhniy-Novgorod Affiliate “ImBio”

A M Nikolaeva

FSUC “SIC Microgen” of MH RF Perm Affiliate «Biomed»

Email: a.m.nikolaeva@mail.ru

T I Glotova

Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: t-glotova@mail.ru
Russian Academy of Agricultural Science

O V Kungurtseva

Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: k-olga-83@mail.ru
Russian Academy of Agricultural Science

A G Glotov

Institute of Experimentally Veterinary Medicine of Siberia and Far East

Email: glotov_vet@mail.ru
Russian Academy of Agricultural Science

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Copyright (c) 2013 Казьянин А.В., Зубкова Н.В., Красильников И.В., Лобастова А.К., Анастасиев В.В., Николаева А.М., Глотова Т.И., Кунгурцева О.В., Глотов А.Г.

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