Особенности пролиферации и апоптоза овариальных фолликулов после локального облучения электронами и введения плазмы, обогащенной тромбоцитами

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Introduction

One of the most unfavorable consequences of radiotherapy for malignant neoplasms of the pelvic organs is irradiation of the ovaries, which can lead to the radiation-i nduced ovarian failure and, as a consequence, infertility [1].

The ovary is very radiosensitive organ [2]. At the molecular level, electron exposure leads to the direct (single and double DNA breaks/crosslinks, chromosomal mutations) and indirect (generation of reactive oxygen (ROS) and nitrogen (RNS) species and lipid peroxidation products) pathomechanisms activation. The life cycle of oocytes is regulated by proliferation (Ki-67), apoptosis (caspase 3) and proapoptosis (p53) factors [3, 4]. There is no effective substrate that can prevent radiation-i nduced apoptosis in ovarian structures for today, and therefore research into new potential radioprotectors remains relevant.

One of the popular regenerative substrates is platelet-rich plasma (PRP) with a large number of biologically active substances containing in the platelets’ α-granules: insulin-like growth factor-1 (IGF-1), platelet- derived growth factor (PDGF), transforming growth factor‑β (TGF‑β1), vascular endothelial growth factor (VEGF), fibroblasts growth factor (FGF), interleukin‑8, fibronectin, etc. [5]. These molecules promote tissue repair and remodeling by initiating cell proliferation, angiogenesis, chemotaxis, re-epithelization, extracellular matrix synthesis, etc.

A team of authors at the annual conference of the European Society of Human Reproduction and Embryology presented the results of using PRP as a regenerative substrate in gynecology [6]. Intraovarian administration of PRP in women with perimenopause, premature ovarian failure and poor ovarian response to in vitro fertilization led to morphofunctional restoration of the ovaries with stabilization of anti-müllerian hormone (AMH), follicle stimulating hormone (FSH) levels and the number of antral follicles within three months after treatment.

Based on the listed positive effects, it is possible to take a PRP as a regenerative substrate for research not only for wounds healing [7], but also in the radiation-i nduced damage treatment in certain organs such as the ovary [8, 9].

It is necessary to conduct research in this direction in order to determine the dose-dependent effects of electron irradiation on the proliferation / apoptosis ratio of oocytes and granulosa cells, and to assess the risks of developing radiation- induced ovarian failure. Such work is also necessary to determine the optimal doses of electron therapy for pelvic organs cancer to level out radiation damage. Particularly important are the search and development of substances with radioprotective properties.

The aim of this study: immunohistochemical analysis of ovarian structures’ cell cycle after administration of platelet-rich plasma in a model of radiation-i nduced ovarian failure.

Materials and methods

Animals for in vivo study

For this research we divided the experimental animals (Wistar rats; n=40) into four groups: Group I (n=10) — control;

Group II (n=10) — fractional local irradiation with electrons in a summary dose (SD) of 20 Gy;

Group III (n=10) — intraperitoneal administration of leukocyte-poor platelet-rich plasma (LP-PRP) 1 hour before local electron irradiation in a SD of 20 Gy;

Group IV (n=10) — intraperitoneal administration of LP-PRP.

High doses of ketamine + xylazine (i/p) used for animal removing on the 7^th day and the first experimental day was the day of the last fraction. All manipulations were kept according to the standard rules: Declaration of Helsinki of the World Medical Association and “International Guidelines for Biomedical Research Using Animals” and approved by the protocol No. 25 of 11/10/23 of the Local Ethics Committee of the National Medical Radiological Research Center.

Morphological study

The ovaries were cut parallel to the sagittal plane (2 mm) and fixed in 10 % formaline after extraction. Then the processing (tissue histological processing apparatus, Leica Biosystems, Germany) were kept under standard conditions and tissues were embedded in paraffin from which serial sections were made (3 μm thick). Micropreparations were deparaffinized, dehydrated and then stained for morphological research with hematoxylin and eosin.

Morphological examination was carried out at a magnification of ×400 in 10 randomly selected fields of view of the light microscope in 5 random sections per sample. Digital scanned preparations were obtained using a video microscopy system (Leica DM3000 microscope, Germany; DFC450 C camera) and image processing software (Leica Application Suite V. 4.9.0) for morphometric analysis.

Immunohistochemical (IHC) study

IHC staining was performed according to the standard manufacturer’s protocols with monoclonal antibodies to Ki-67 (ThermoFisher, Clone MM1) and Caspase 3 (ThermoFisher, Clone 74T2) as a primary antibodies [10, 11]. A universal two-component HiDef Detection™ HRP Polymer system (Cell Marque, USA), mouse/rabbit anti- IGG, horseradish peroxidase (HRP) and DAB substrate were used to determine secondary antibodies. Antigen unmasking was carried out in a citrate buffer with pH≈6.0 in a water bath with a pT Link microprocessor (Dako, Denmark) at a temperature of 95ºС for 40 minutes and then by cooling at a temperature of 20ºС for 20 minutes. For cell nuclei counterstaining a Mayer’s hematoxylin was used. The number of IHC positively stained cells (in %) was counted at a magnification of ×400 in 10 randomly fields of view.

Statistical analysis

For analytical processing of the research results, the Windows package SPSS 12 (IBM Analytics, USA) statistical program was used. The obtained data are presented as mean ± standard error. Comparisons between groups were carried out using statistical packages and differences were considered significant at p-value <0.05.

Results and discussion

In animals of the control and IV groups, the ovary is covered on the outside with single- layer squamous epithelium (mesothelium), deeper — the tunica albuginea, formed by dense fibrous connective tissue with cords extending from it. The ovarian parenchyma is represented by numerous follicles in different stages of development (Fig. 1).

Fig. 1. Ovaries in experimental groups; hematoxylin and eosin stain, magn. ×200
Note: SD — summary dose, LP-PRP — leukocyte poor platelet-rich plasma.

Fractional local electron irradiation led to the radiation- induced ovarian failure within a week. The number of primordial follicles was sharply reduced, and the number of atretic follicles was increased. In some follicles, oocytes with signs of pyknosis, fragmentation of granulosa cells, and cellular detritus in the antrum were noted. In the stroma of the organ, multiple hemorrhages, stasis of most blood vessels, and proliferation of connective tissue were found compared to the control (Fig. 1).

Administration of platelet-rich plasma before irradiation led to a decrease in the degree of radiation- induced ovarian damage compared to the morphological pattern of group II: the primordial and primary follicles counts was slightly reduced unevenly distributed over the area of the ovary; isolated hemorrhages and stasis of red blood cells in vessels’ lumen (Fig. 1).

An immunohistochemical study of ovaries irradiated with a summary dose of 20 Gy revealed a decrease in Ki-67 expression level in the follicles (3.9 times) and the corpus luteum (1.5 times), while the proportion of Ki-67-positive theca cells sharply increased (7.5 times) compared to the control group. Number of caspase-3-immunopositive granulosa cells increased 3.8 times, while practically no differences in the corpus luteum and theca cells were observed compared to the control (Fig. 2–4).

Administration of LP-PRP in group III led to a partial restoration of the proliferative activity of granulosa cells (2.4 times) compared to the irradiation group, however, the proportion of Ki-67-positive theca cells decreased (1.3 times) compared to Group II. A significant decrease (1.6 times) in the expression of caspase-3 in group III was observed only in granulosa cells compared to the results of the irradiation group.

Although, no statistically significant differences were found between the levels of immunoreactivity in group IV compared to the control.

Fig. 2. Count of Ki‑67‑positive cells in experimental ovaries
Note: GC — granulosa cells, CL — corpus luteum, TC — theca cells, SD — summary dose. For comparison the Kruskal- Wallis test and the Mann-W hitney U test were performed; * — significant differences SD 20 Gy vs control (p <0.05). ** — significant differences SD 20 Gy vs SD 20 Gy + LP-PRP (p <0.05).

Fig. 3. Count of caspase‑3‑positive cells in experimental ovaries
Note: GC — granulosa cells, CL — corpus luteum, TC — theca cells, SD — summary dose. For comparison the Kruskal- Wallis test and the Mann- Whitney U test were performed. * — significant differences SD 20 Gy vs control (p <0.05). ** — significant differences SD 20 Gy vs SD 20 Gy + LP-PRP (p <0.05).

Fig. 4. Immunohistochemical analysis of Ki‑67 and caspase‑3 expression in experimental ovaries, magn. ×400

Local electron irradiation in group II led to a marked decrease in the Ki-67 expression in granulosa and corpus luteum cells in combination with a sharp increase of caspase-3-stained follicular cells. This is probably because of the effect of electrons on proliferatively active cells which lead to both direct (single and double DNA damages and chromosomal mutations) and indirect (ROS and RNS generation, lipid peroxidation and molecular water radiolysis) activation of pathways including in post-radiation toxicity in ovaries [12]. Then it leads to a strongly decrease in the primordial follicles number, fibrous connective tissue overgrowth which results in radiation- induced premature ovarian failure with ovarian reserve decrease, early menopause and infertility [13].

Post-radiation cell death most often occurs by apoptosis with activation of the cytochrome c and caspase cascade pathways, and in our study, granulosa cells turned out to be the most sensitive to the effects of electron irradiation, which was accompanied by a sharp induction of their apoptosis, confirmed by a high level of caspase-3 expression. Almost similar results were obtained by other researchers [14]. In our opinion a secretory disruption in granulosa cells results to the decreased synthesis of steroid hormones and growth factors. These changes led to the secondary violation of ovofolliculogenesis. In addition, theca cells hyperplasia is also responsible for the secretory follicular dysfunction, and it is the compensatory response to decreased hormone levels in blood.

Due to the fact that electrons lead to a decrease in the synthesis of key growth factors responsible for the restoration and regeneration in ovary, it was advisable to use platelet‑rich plasma where the platelets’ α‑granules contain high concentrations of biologically substances capable of inducing the follicular cells’ regenerative activity and metabolism (through neoangiogenesis stimulation) [15, 16]. Thus, the most important of these in PRP are IGF-1, PDGF, TGF-β1, VEGF, FGF, interleukin‑8, fibronectin, etc. [17]

These biologically active molecules are the key factors in proliferation and differentiation of many cell types. Due to this it can restore proliferative- apoptotic balance, probably responsible for the regenerative and radioprotective LP-PRP properties discovered in the present study: higher levels of proliferative activity of granulosa cells combined with significantly lower rates caspase‑3 immunoreactivity compared with the irradiation group. In addition, pre-irradiation administration of LP-PRP led to a less pronounced increase in the theca cells proliferation, which may indirectly indicate the body’s low need for compensatory-a daptive hyperplasia of these cells and the preservation of close to physiological levels of steroid hormones.

Thus, based on histological and immunohistochemical studies, it was occured that pre-radiation administration of LP-PRP contributed to the restoration of the proliferation/apoptosis ratio of follicles, which does not exclude the protective effect of this regenerative substrate, which is especially important for the prevention of the development of radiation- induced ovarian failure.

Conclusion

Components of platelet-rich plasma have radioprotective properties, maintaining the cell cycle of follicular cells and reducing the depth and range of radiation damage to the ovary after 20 Gy electron exposure, confirmed by the Ki‑67 and caspase 3 expression levels.

Об авторах

Григорий Демяшкин

Российский университет дружбы народов имени Патриса Лумумбы; Национальный медицинский исследовательский центр радиологии

Email: dr.dga@mail.ru
ORCID iD: 0000-0001-8447-2600
SPIN-код: 5157-0177

док. мед. наук, ведущий научный сотрудник Научно-образовательного ресурсного центра «Инновационные технологии иммунофенотипирования, цифрового пространственного профилирования и ультраструктурного анализа» Российского университета дружбы народов имени Патриса Лумумбы, заведующий отделом патоморфологии Национального медицинского исследовательского центра радиологии Минздрава РФ

Россия, 117198, Россия, Москва, ул. Миклухо-Маклая, 6; 125284, Россия, Москва, 2-й Боткинский проезд, д. 3

Заира Муртазалиева

Российский университет дружбы народов

Email: ZARIA.ALIEVA.90@BK.RU
ORCID iD: 0009-0000-2361-7618
SPIN-код: 2847-2984

аспирант ИТМиБ Сеченовского университета

Россия, г. Москва, Российская Федерация

Екатерина Пугачева

Российский университет дружбы народов

Email: rouzella@mail.ru
ORCID iD: 0009-0009-2268-3838
SPIN-код: 5784-9384

аспирант ИТМиБ Сеченовского университета

Россия, г. Москва, Российская Федерация

Матвей Анатольевич Вадюхин

Первый Московский государственный университет им. И.М. Сеченова

Email: vma20@mail.ru
ORCID iD: 0000-0002-6235-1020
SPIN-код: 9485-7722

студент Института клинической медицины им. Н.В. Склифосовского

Россия, 119991, Россия, Москва, ул. Трубецкая, 8/2

Макка Бимурзаева

Первый Московский государственный университет им. И.М. Сеченова

Email: bimakka@mail.ru
ORCID iD: 0000-0002-3065-0755
SPIN-код: 2637-5894

студент Института клинической медицины им. Н.В. Склифосовского

119991, Россия, Москва, ул. Трубецкая, 8/2

Айгуль Милованова

Первый Московский государственный университет им. И.М. Сеченова

Email: Ovezberdiyevaayka@gmail.com
ORCID iD: 0009-0007-9681-5704
SPIN-код: 9590-8578

студент Института клинической медицины им. Н.В. Склифосовского

Россия, 119991, Россия, Москва, ул. Трубецкая, 8/2

Тамара Деньгина

Первый Московский государственный университет им. И.М. Сеченова

Автор, ответственный за переписку.
Email: larobrine@mail.ru
ORCID iD: 0009-0002-0651-9711
SPIN-код: 5877-3869

студент Института клинической медицины им. Н.В. Склифосовского

119991, Россия, Москва, ул. Трубецкая, 8/2

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