Carboxypeptidase A3 in the structure of the protease phenotype of mast cells: cytophysiological aspects

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Abstract

Carboxypeptidase A3 (CPA3) is a specific protease of mast cells (MC) with variable expression and appears to be one of the preformed components of the secretome. CPA3 is involved in regulation of the state of a specifi tissue microenvironment and components of the integrative-buffer metabolic environment in adaptive and pathological processes; it affects implementation of the innate immunity, mechanisms of angiogenesis, processes of the extracellular matrix remodeling, etc. CPA3 identification using protocols of multiplex immunohistochemistry allows specifying details of the organ-specific mast cell population features, including the protease phenotype, mechanisms of biogenesis with cytoand histotopographic criteria, and features of secretory pathways. Numerous biological effects of CPA3, including participation in the regulation of the pulmonary parenchyma and systemic blood flow, in biogenesis and remodeling of the fibrous component of the extracellular matrix, in epigenetic reprogramming, determine the importance of fundamental investigation of the physiological activity of protease and its involvement in the implementation of pathological processes. Further studies will contribute to the detection of the translational value of the mast cell CPA3 expression features as a prognostic factor and a promising molecular target for treatment of socially significant diseases.

About the authors

Dmitrii A. Atiakshin

Peoples’ Friendship University of Russia; Voronezh N.N. Burdenko State Medical University

Author for correspondence.
Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0002-8347-4556
Moscow, Russian Federation; Voronezh, Russian Federation

Andrey A. Kostin

Peoples’ Friendship University of Russia

Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0002-0792-6012
Moscow, Russian Federation

Ivan D. Trotsenko

Peoples’ Friendship University of Russia

Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0002-6667-0125
Moscow, Russian Federation

Victoria V. Shishkina

Voronezh N.N. Burdenko State Medical University

Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0001-9185-4578
Voronezh, Russian Federation

Markus Tiemann

Institute for Hematopathology

Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0002-6499-4855
Hamburg, Germany

Igor B. Buchwalow

Institute for Hematopathology

Email: atyakshin-da@rudn.ru
ORCID iD: 0000-0003-1142-7483
Hamburg, Germany

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