RUDN Journal of MedicineRUDN Journal of MedicineВестник Российского университета дружбы народов. Серия: Медицина2313-02452313-0261Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)3053310.22363/2313-0245-2022-26-1-9-33PhysiologyФизиологияReview ArticleCarboxypeptidase A3 in the structure of the protease phenotype of mast cells: cytophysiological aspectsКарбоксипептидаза А3 в структуре протеазного фенотипа тучных клеток: цитофизиологические аспектыhttps://orcid.org/0000-0002-8347-4556AtiakshinDmitrii A.АтякшинД. А.atyakshin-da@rudn.ruhttps://orcid.org/0000-0002-0792-6012KostinAndrey A.КостинА. А.atyakshin-da@rudn.ruhttps://orcid.org/0000-0002-6667-0125TrotsenkoIvan D.ТроценкоИ. Д.atyakshin-da@rudn.ruhttps://orcid.org/0000-0001-9185-4578ShishkinaVictoria V.ШишкинаВ. В.atyakshin-da@rudn.ruhttps://orcid.org/0000-0002-6499-4855TiemannMarkusТиманнМ.atyakshin-da@rudn.ruhttps://orcid.org/0000-0003-1142-7483BuchwalowIgor B.БухваловИ. Б.atyakshin-da@rudn.ruPeoples’ Friendship University of RussiaРоссийский университет дружбы народовVoronezh N.N. Burdenko State Medical UniversityВоронежский государственный медицинский университет имени Н.Н. БурденкоInstitute for HematopathologyИнститут гематопатологии21032022261PHYSIOLOGYФИЗИОЛОГИЯ93321032022Copyright ©; 2022, Atiakshin D.A., Kostin A.A., Trotsenko I.D., Shishkina V.V., Tiemann M., Buchwalow I.B.Copyright ©; 2022, Атякшин Д.А., Костин А.А., Троценко И.Д., Шишкина В.В., Тиманн М., Бухвалов И.Б.2022Atiakshin D.A., Kostin A.A., Trotsenko I.D., Shishkina V.V., Tiemann M., Buchwalow I.B.Атякшин Д.А., Костин А.А., Троценко И.Д., Шишкина В.В., Тиманн М., Бухвалов И.Б.http://creativecommons.org/licenses/by/4.0https://journals.rudn.ru/medicine/article/view/30533

Carboxypeptidase A3 (CPA3) is a specific protease of mast cells (MC) with variable expression and appears to be one of the preformed components of the secretome. CPA3 is involved in regulation of the state of a specifi tissue microenvironment and components of the integrative-buffer metabolic environment in adaptive and pathological processes; it affects implementation of the innate immunity, mechanisms of angiogenesis, processes of the extracellular matrix remodeling, etc. CPA3 identification using protocols of multiplex immunohistochemistry allows specifying details of the organ-specific mast cell population features, including the protease phenotype, mechanisms of biogenesis with cytoand histotopographic criteria, and features of secretory pathways. Numerous biological effects of CPA3, including participation in the regulation of the pulmonary parenchyma and systemic blood flow, in biogenesis and remodeling of the fibrous component of the extracellular matrix, in epigenetic reprogramming, determine the importance of fundamental investigation of the physiological activity of protease and its involvement in the implementation of pathological processes. Further studies will contribute to the detection of the translational value of the mast cell CPA3 expression features as a prognostic factor and a promising molecular target for treatment of socially significant diseases.

Карбоксипептидаза А3 (СРА3) является специфической протеазой тучных клеток (ТК) с вариабельной экспрессией и входит в число преформированных компонентов секретома. CPA3 принимает участие в регуляции состояния специфического тканевого микроокружения и компонентов интегративно-буферной метаболической среды при адаптивных и патологических процессах, затрагивая реализацию врожденного иммунитета, механизмы ангиогенеза, процессы ремоделирования межклеточного матрикса и др. Идентификация СРА3 c помощью протоколов мультиплексной иммуногистохимии позволяет конкретизировать детали органоспецифических популяционных характеристик тучных клеток, включая протеазный фенотип, механизмы биогенеза с цитои гистотопографическими критериями, а также особенности секреторных путей. Многочисленные биологические эффекты СРА3, включая участие в регуляции состояния легочной паренхимы и системного кровотока, в биогенезе и ремоделировании волокнистого компонента внеклеточного матрикса, в эпигенетическом репрограммировании, определяют важность фундаментальных исследований физиологической активности протеазы и ее вовлеченности в реализацию патологических процессов. Дальнейшие исследования будут способствовать раскрытию трансляционного значения характеристик экспрессии CPA3 ТК в качестве прогностического фактора и перспективной молекулярной мишени терапии социально значимых заболеваний.

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