Influence of glial progenitor cells on the restoration of sensorimotor deficits in rats after traumatic brain injury

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Abstract

Relevance. The search for new methods of effective therapy for traumatic brain injury is one of the important tasks of modern biomedicine. One promising approach for treating traumatic brain injury is cell therapy. The aim of the work is to study the therapeutic effect of glial progenitor cells derived from induced pluripotent stromal cells in an experimental model of traumatic brain injury. Materials and Methods. Modeling of traumatic brain injury was carried out on mature male Wistar rats. The therapeutic group was administered a single dose of 750*103 cells/ml glial progenitor cells with a volume of 1 ml, and the control group — 1 ml of phosphate-­buffered saline. Administration was carried out intra-­arterially 24 hours after injury. To analyze the therapeutic effectiveness, an MRI study was performed on the 14th day, as well as a limb-placing test on the 1st, 3rd, 7th and 14th days. Histological examination was carried out on days 1, 3 and 7 after administration to assess the migration and distribution of stained cells (concentration 750*103 cells/ml) by lipophilic dye PKH26 (Sigma, USA) at the rat’s brain tissues after traumatic brain injury. Measurements of injury volume and counts of PKH26-stained cells were performed using ImageJ software (Wayne Rasband, National Institute of Mental Health, Bethesda, MD, USA). The statistical analysis was carried out using GraphPad Prism 8.2.0 program (GraphPad Software, Inc., USA). Results and Discussion. Administration of GPCs led to decreasing the damage volume. Significant decrease in sensorimotor deficit was observed on days 3, 7 and 14 after injury compared with the control group. Intra-arterial administration resulted in successful delivery of glial progenitor cells to brain tissue. Cells were detected in the cerebral cortex, hippocampus, and striatum on day 1, and were not observed on days 3 and 7 after administration. Conclusion. Intra-arterial administration of GPCs leads to efficient migration of cells into brain tissue. Glial progenitor cells therapy promotes neurorecovery processes after traumatic brain injury. This therapy is a promising treatment for traumatic brain injury.

About the authors

Anastasiia K. Sudina

Research Centre for Medical Genetics

Email: nastyasudina@gmail.com
ORCID iD: 0000-0003-3531-7684
SPIN-code: 5225-7878
Moscow, Russian Federation

Mikhail E. Ivanov

Lomonosov Moscow State University

Email: nastyasudina@gmail.com
ORCID iD: 0000-0002-5010-3919
SPIN-code: 8333-9897
Moscow, Russian Federation

Alexander M. Yurin

Lomonosov Moscow State University

Email: nastyasudina@gmail.com
ORCID iD: 0009-0000-9909-0971
Moscow, Russian Federation

Andrey V. Makarov

Pirogov Russian National Research Medical University

Email: nastyasudina@gmail.com
ORCID iD: 0000-0002-5847-567X
SPIN-code: 3534-3764
Moscow, Russian Federation

Timur Kh. Fatkhudinov

Avtsyn Research Institute of Human Morphology of FSBSI «Petrovsky National Research Centre of Surgery»

Email: nastyasudina@gmail.com
ORCID iD: 0000-0002-6498-5764
SPIN-code: 7919-8430
Moscow, Russian Federation

Dmitry V. Goldstein

Research Centre for Medical Genetics

Email: nastyasudina@gmail.com
ORCID iD: 0000-0003-2438-1605
SPIN-code: 7714-9099
Moscow, Russian Federation

Diana I. Salikhova

Avtsyn Research Institute of Human Morphology of FSBSI «Petrovsky National Research Centre of Surgery»

Author for correspondence.
Email: nastyasudina@gmail.com
ORCID iD: 0000-0001-7842-7635
SPIN-code: 1436-5027
Moscow, Russian Federation

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Supplementary files

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1. Fig.1. Representative MRI images of the damage volume during modeling of TBI on the 14th day of the experiment.

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2. Fig. 2. Histological study of GPCs migration in the brain

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Copyright (c) 2024 Sudina A.K., Ivanov M.E., Yurin A.M., Makarov A.V., Fatkhudinov T.K., Goldstein D.V., Salikhova D.I.

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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

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