Chlorella growth stimulation depending on the duration of extremely high frequencies electromagnetic radiation exposure

Cover Page

Cite item

Abstract

The result indicates the possibility of creating methods to stimulate the growth rate of microalgae by extremely high frequency electromagnetic radiation (EHF EMF). Pollution of quarry waste water with inorganic nitrogen compounds (ammonium, nitrate, nitrite) is an important problem in the mining industry. Biological treatment methods with the use of plant organisms and microorganisms show efficiency and require less financial and labour costs than physical and chemical methods of waste water treatment. Not all organisms and microorganisms are applicable in the Far North conditions. The microalgae Chlorella shows the ability to reduce nitrate and ammonium concentrations in quarry wastewater, and some species of microalgae can survive at water temperatures of 3⁰C. A number of works indicate the stimulating effect of EHF EMR on microorganisms and the ability to reduce the toxicity of pollutants. Therefore, the methods of treatment of quarry waste water from inorganic nitrogen compounds by microalgae Chlorella under the influence of electromagnetic radiation of extremely high frequency are relevant for development. The aim of the study was to investigate the effect of the duration of EHF EMR exposure on the growth rate of the Chlorella microalgae. Materials and Methods: In this work, 2 control and 9 working experiments were carried out to cultivate Chlorella vulgaris for 24 hours with the exposure time of EMF EHF from 5 to 480 minutes at the beginning of cultivation. The increase in biomass concentration in the experiments relative to control values was studied spectrophotometrically. Results: The maximum increase in biomass concentration was observed at an exposure time of 120 minutes.

About the authors

Gleb A. Shcheglov

Kola Science Center, Russian Academy of Science (INEP KSC RAS)

Author for correspondence.
Email: g.scheglov@ksc.ru
ORCID iD: 0000-0003-2870-3616

Engineer, Institute of North Industrial Ecology Problems

14a Fersman St, Apatity, Murmansk region, 184209, Russian Federation

References

  1. Khokhryakov AB, Studenok AG, Olkhovsky AM, Studenok GA. Quantitative assessment of the contribution of blasting operations to the pollution of drainage waters of quarries with nitrogen compounds. Proceedings of Higher Educational Institutions. Mining Journal. 2005;6:29–31. (In Russ.). Available from: https://www.elibrary.ru/ item.asp?id=11642420 (accessed: 07.11.2022).
  2. Khokhryakova AV, Studenok AG, Studenok GA. Investigation of the processes of formation of chemical pollution of drainage waters with nitrogen compounds on the example of a quarry of a large mining enterprise. Proceedings of the Ural State Mining University. 2016;4(44):35–37. (In Russ.). http://doi.org/10.21440/2307-2091-2016-4-35-37
  3. Babatunde AO, Zhao YQ, Doyle RJ, Rackard SM, Kumar JLG, Hu YS. On the fit of statistical and the k-C * models to projecting treatment performance in a constructed wetland system. Environ Sci Health A Tox Hazard Subst. 2011;46(5):490-9. http://doi.org/10.1080/10934529.2011.551729
  4. Kirilina TV, Do Thi Thu Hang, Sirotkin AS. Evaluation of the efficiency of wastewater treatment using unicellular and multicellular hydrobionts. Bulletin of Kazan Technological University. 2013;8(16):200–203. (In Russ.)
  5. Solnyshkova MA. Reduction of surface water pollution by inorganic nitrogen compounds in the impact zone of mining enterprises of the Murmansk region (dissertation of the Candidate of Technical Sciences). Saint Petersburg; 2020. (In Russ.). Available from: https://goo.su/N55RjSd (accessed: 07.11.2022).
  6. Li ZY, Guo SY, Li L, Cai MY. Effects of electromagnetic field on the batch cultivation and nutritional composition of Spirulina platensis in an air-lift photobioreactor. Bioresour. Technol. 2007;98(3):700–705.
  7. Sukhovsky NA. The presence of an electrostatic field in an electrostatic bioreactor. Bulletin of the Agroindustrial complex of the Upper Volga region. 2015;(1):92–94. (In Russ.)
  8. Gapochka MG. Ecological aspects of interaction of electromagnetic fields of the millimeter range with biological objects (dissertation of the Doctor of Biological Sciences). Moscow; 2013. (In Russ.). Available from: https://www.elibrary.ru/ item.asp?id=30397922 (accessed: 27.09.2022).
  9. Gapochka MG. The influence of low-intensity electromagnetic radiation on the toxicity of the aquatic environment. VMU physics astronomy. 2009;1:67–69. (In Russ.)
  10. Zarubina AP, Gapochka MG, Novoselova LA, Gapochka LD. Biotesting by the ecolume test system of the effect of a low-intensity electromagnetic field on the toxicity of household wastewater. Bulletin of the Moscow University. Biology. 2012;16(3):39–43. (In Russ.)
  11. Shcheglov GA, Masloboev VA. Prospects of the Chlorella application for the nitrogen quarry waters purification at low temperature. Proceedings of the Fersman scientific session of the GI KNC RAS. 2022;19:424–429. (In Russ.). http://doi.org/10.31241/FNS.2022.19.077

Copyright (c) 2023 Shcheglov G.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies