Changes in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the water


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Abstract

The aim of the research was to study the effect of the water menu with a low D/H on morphological hepatocytes parameters in rabbits in modeling of an acute hypoxic liver damage. Methods: morphological research of liver tissues of the experimental animals had been done by cytological and histological methods, which had been prepared impression smear of the studying organ after which it had been stained with azur-eosin according to a common procedure; the blood plasma had been done by the pulsing nuclear magnetic resonance spectrometer JEOL JNM-ECA 400MHz, evaluate an isotopic composition of rat lyophilized liver tissues had been used an isotopic mass-spectrometer DELTAplus. Results: There are the statistics presented in the given study reveal the feature of the water menu with the low deuterium concentration decreases the liver tissue damage in hypoxia simulating by the compression of a hepatoduodenal ligament. It was demonstrated that the use of the deuterium depleted water (DDW) reliably decreases the deuterium concentration in a blood plasma (by 32%) and in liver tissue (by 9%), which shows the effectiveness of the correlation of isotopic composition of internal environment and organs by the products with the low deuterium concentration. Conclusion: It has been established that the use of the DDW as a hepatoprotective measure is equal in its effectiveness to some of lipophilic antioxidants. This allows to use vegetative antioxidant factors and a water menu with a low deuterium concentration (40 ppm) as a combined measure, in order to correct morphological liver defects in its ischemic damage.

About the authors

M I Bykov

Kuban state medical university; Scientific research institute of the Ochapovsky regional clinic of Ministry of Health of the Russian Federation

Email: bikov_mi@mail.ru
M.D., Ph.D., Associate Professor of the Department of Surgery №1, State Educational Institution of Higher Professional Learning “The Kuban State Medical University” of the Ministry of Public Health of Russia, physician in chief of the Department of Endoscopy № 2, State Educational Institution of Learning “Scientific Research Institute of the Ochapovsky Regional Clinic” of the Ministry of Public Health of Russia.

M Chikhladze

National Institute of Allergology, Asthma and Clinical Immunology of Georgian National Academy of Science

Email: bikov_mi@mail.ru

V V Shchava

Scientific research institute of the Ochapovsky regional clinic of Ministry of Health of the Russian Federation

Email: bikov_mi@mail.ru

A A Elkina

Kuban state university

Email: bikov_mi@mail.ru

I M Bykov

Kuban state medical university

Email: bikov_mi@mail.ru

K A Popov

Kuban state medical university

Email: bikov_mi@mail.ru

A V Moiseev

Kuban state agrarian university

Email: bikov_mi@mail.ru

M G Baryshev

Kuban state university

Email: bikov_mi@mail.ru

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Copyright (c) 2018 Bykov M.I., Chikhladze M., Shchava V.V., Elkina A.A., Bykov I.M., Popov K.A., Moiseev A.V., Baryshev M.G.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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