RUDN Journal of MedicineRUDN Journal of MedicineВестник Российского университета дружбы народов. Серия: Медицина2313-02452313-0261Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)1844610.22363/2313-0245-2018-22-1-120-126EXPERIMENTAL PHYSIOLOGYЭКСПЕРИМЕНТАЛЬНАЯ ФИЗИОЛОГИЯResearch ArticleChanges in rabbits’ liver morphological parameters in simulation of hypoxia corrected by the use of the modified isotopic D/H composition in the waterИзменение морфологических показателей печени у кроликов при моделировании гипоксии, корригируемой потреблением питьевого рациона с модифицированным изотопным D/H составомBykovM IБыковМ ИM.D., Ph.D., Associate Professor of the Department of Surgery №1, State Educational Institution of Higher Professional Learning “The Kuban State Medical University” of the Ministry of Public Health of Russia, physician in chief of the Department of Endoscopy № 2, State Educational Institution of Learning “Scientific Research Institute of the Ochapovsky Regional Clinic” of the Ministry of Public Health of Russia.bikov_mi@mail.ruChikhladzeMЧихладзеМbikov_mi@mail.ruShchavaV VЩаваВ Вbikov_mi@mail.ruElkinaA AЕлкинаА Аbikov_mi@mail.ruBykovI MБыковИлья Михайловичд.м.н., профессор, заведующий кафедрой фундаментальной и клинической биохимии, Кубанский государственный медицинский университетbikov_mi@mail.ruPopovK AПоповК Аbikov_mi@mail.ruMoiseevA VМоисеевА Вbikov_mi@mail.ruBaryshevM GБарышевМ Гbikov_mi@mail.ruKuban state medical universityФедеральное государственное бюджетное образовательное учреждение высшего образования «Кубанский государственный медицинский университет» Министерства здравоохранения Российской ФедерацииScientific research institute of the Ochapovsky regional clinic of Ministry of Health of the Russian FederationГБУЗ «Научно-исследовательский институт - Краевая клиническая больница № 1 имени профессора С.В. Очаповского» Министерства здравоохранения Краснодарского краяNational Institute of Allergology, Asthma and Clinical Immunology of Georgian National Academy of ScienceНациональный Институт Аллергологии, астмы и клинической иммунологии Национальной Академии Наук ГрузииKuban state universityФедеральное государственное бюджетное образовательное учреждение высшего образования «Кубанский государственный университет»Kuban state agrarian universityФедеральное государственное бюджетное образовательное учреждение высшего образования «Кубанский государственный университет»15122018221VOL 22, NO1 (2018)ТОМ 22, №1 (2018)12012601052018Copyright ©; 2018, Bykov M.I., Chikhladze M., Shchava V.V., Elkina A.A., Bykov I.M., Popov K.A., Moiseev A.V., Baryshev M.G.Copyright ©; 2018, Быков М.И., Чихладзе М., Щава В.В., Елкина А.А., Быков И.М., Попов К.А., Моисеев А.В., Барышев М.Г.2018Bykov M.I., Chikhladze M., Shchava V.V., Elkina A.A., Bykov I.M., Popov K.A., Moiseev A.V., Baryshev M.G.Быков М.И., Чихладзе М., Щава В.В., Елкина А.А., Быков И.М., Попов К.А., Моисеев А.В., Барышев М.Г.http://creativecommons.org/licenses/by/4.0https://journals.rudn.ru/medicine/article/view/18446The aim of the research was to study the effect of the water menu with a low D/H on morphological hepatocytes parameters in rabbits in modeling of an acute hypoxic liver damage. Methods: morphological research of liver tissues of the experimental animals had been done by cytological and histological methods, which had been prepared impression smear of the studying organ after which it had been stained with azur-eosin according to a common procedure; the blood plasma had been done by the pulsing nuclear magnetic resonance spectrometer JEOL JNM-ECA 400MHz, evaluate an isotopic composition of rat lyophilized liver tissues had been used an isotopic mass-spectrometer DELTAplus. Results: There are the statistics presented in the given study reveal the feature of the water menu with the low deuterium concentration decreases the liver tissue damage in hypoxia simulating by the compression of a hepatoduodenal ligament. It was demonstrated that the use of the deuterium depleted water (DDW) reliably decreases the deuterium concentration in a blood plasma (by 32%) and in liver tissue (by 9%), which shows the effectiveness of the correlation of isotopic composition of internal environment and organs by the products with the low deuterium concentration. Conclusion: It has been established that the use of the DDW as a hepatoprotective measure is equal in its effectiveness to some of lipophilic antioxidants. This allows to use vegetative antioxidant factors and a water menu with a low deuterium concentration (40 ppm) as a combined measure, in order to correct morphological liver defects in its ischemic damage.В работе представлены данные, свидетельствующие о способности питьевого рациона с пониженным содержанием дейтерия уменьшать повреждение тканей печени при моделировании гипоксии путем пережатия гепатодуоденальной связки. Показано, что потребление deuterium depleted water (DDW) достоверно уменьшает концентрации дейтерия в плазме крови (на 32%) и в тканях печени (на 9%), что свидетельствует об эффективности коррекции изотопного состава внутренних сред и органов с помощью продуктов с пониженным содержанием дейтерия. Также установлено, что использование DDW с гепатопротекторными целями по своей эффективности не уступает некоторым антиоксидантам липофильного происхождения. Все это позволяет проводить сочетанное использование растительных антиоксидантных факторов и питьевого рациона с пониженным содержанием дейтерия (40 ppm) для коррекции морфологических повреждений печени при ее ишемическом поражении.deuteriumliverrabbitsmorphologyhypoxiaдейтерийпеченькроликиморфологиягипоксия1.Belyakin S.А., Botrov A.N., Plyusnin S.V. The role of liver biopsy in the diagnosis of alcoholic hepatitis. Military medical journal. 2011. V. 5. 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