Despite the upsurge in conflicts at the turn of the 20th and 21st centuries and the increase in peacekeeping activities, women’s participation in peacekeeping operations has been the exception to the rule rather than the standard practice. However, the UN has focused on the negative and disproportional impact of internal armed conflicts on women and children. For the past two decades, the UN has been working to incorporate a gender perspective into the peacekeeping architecture. The starting point was UN Security Council Resolution 1325, adopted in 2000, which fully recognized the importance of the gender perspective in peace and security. The UN’s further systematic actions in this direction have changed the simplistic view of women as victims of internal armed conflicts and have recognized them as active contributors to peace and security. The purpose of the article is to comprehensively examine the current state of the international community’s commitment to women, peace and security in order to fill the existing theoretical gaps and find further ways to enhance the role of women in peace and security activities. The study is based on interdisciplinary, historical and structural-functional approaches, and uses content analysis of official UN documents and data, analytical reports of international organizations, relevant scientific materials and publications. The authors conclude that UNSCR 1325 (2000) initiated a major shift in UN peacekeeping in the context of a large-scale “Women, Peace and Security” agenda and has led to new approaches to women’s participation in all stages of peace processes. Women’s active and equal participation in peace negotiations and the implementation of political decisions has become an important factor in strengthening peace and security. Moreover, the UN’s current activities are not limited to women’s participation in peacekeeping. In addition to changes in the planning and staffing of peacekeeping operations, the UN has also taken steps to address less visible behavioral issues, including the prevention of sexual and gender-based violence, as well as the professional skills of peacekeeping personnel. At the same time, the increase in the number of women in the military and police components of UN missions has raised some international concerns about the potential masculinization of female peacekeepers.