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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Structural Mechanics of Engineering Constructions and Buildings</journal-id><journal-title-group><journal-title xml:lang="en">Structural Mechanics of Engineering Constructions and Buildings</journal-title><trans-title-group xml:lang="ru"><trans-title>Строительная механика инженерных конструкций и сооружений</trans-title></trans-title-group></journal-title-group><issn publication-format="print">1815-5235</issn><issn publication-format="electronic">2587-8700</issn><publisher><publisher-name xml:lang="en">Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">31050</article-id><article-id pub-id-type="doi">10.22363/1815-5235-2022-18-1-54-63</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Experimental researches</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Экспериментальные исследования</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Use of natural compounds as a nutrition for bacteria in self-healing mortar</article-title><trans-title-group xml:lang="ru"><trans-title>Использование природных соединений в качестве питания бактерий в самовосстанавливающихся рамах</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-2493-7255</contrib-id><name-alternatives><name xml:lang="en"><surname>Galishnikova</surname><given-names>Vera V.</given-names></name><name xml:lang="ru"><surname>Галишникова</surname><given-names>Вера Владимировна</given-names></name></name-alternatives><bio xml:lang="en"><p>Doctor of Technical Sciences, Professor, Director of the International Department</p></bio><bio xml:lang="ru"><p>доктор технических наук, профессор, директор международного департамента</p></bio><email>galishni@gmail.com</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-6002-3827</contrib-id><name-alternatives><name xml:lang="en"><surname>Elroba</surname><given-names>Sherif M.</given-names></name><name xml:lang="ru"><surname>Эльроба</surname><given-names>Шериф М.</given-names></name></name-alternatives><bio xml:lang="en"><p>PhD Student, Department of Civil Engineering, Academy of Engineering</p></bio><bio xml:lang="ru"><p>аспирант, кафедра гражданского строительства, Инженерная академия</p></bio><email>ERU.SHERIF@yahoo.com</email><xref ref-type="aff" rid="aff2"/><xref ref-type="aff" rid="aff3"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3107-5796</contrib-id><name-alternatives><name xml:lang="en"><surname>Dayoub</surname><given-names>Nbras</given-names></name><name xml:lang="ru"><surname>Даюб</surname><given-names>Нбрас</given-names></name></name-alternatives><bio xml:lang="en"><p>PhD Student, Department of Technology and Organization of Construction Production</p></bio><bio xml:lang="ru"><p>аспирант, кафедра технологии и организации строительного производства</p></bio><email>nbrasdayoub@hotmail.com</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7712-5942</contrib-id><name-alternatives><name xml:lang="en"><surname>Sakna</surname><given-names>Ahmad</given-names></name><name xml:lang="ru"><surname>Сакна</surname><given-names>Ахмад</given-names></name></name-alternatives><bio xml:lang="en"><p>Teaching Assistant, Faculty of Engineering, Construction Engineering Department</p></bio><bio xml:lang="ru"><p>ассистент, кафедра строительной инженерии, инженерный факультет</p></bio><email>ahmadsakna96@gmail.com</email><xref ref-type="aff" rid="aff3"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Moscow State University of Civil Engineering (National Research University)</institution></aff><aff><institution xml:lang="ru">Московский государственный строительный университет</institution></aff></aff-alternatives><aff-alternatives id="aff2"><aff><institution xml:lang="en">Peoples’ Friendship University of Russia (RUDN University)</institution></aff><aff><institution xml:lang="ru">Российский университет дружбы народов</institution></aff></aff-alternatives><aff-alternatives id="aff3"><aff><institution xml:lang="en">Egyptian Russian University (ERU University)</institution></aff><aff><institution xml:lang="ru">Египетско-Российский университет</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2022-05-23" publication-format="electronic"><day>23</day><month>05</month><year>2022</year></pub-date><volume>18</volume><issue>1</issue><issue-title xml:lang="en">VOL 18, NO1 (2022)</issue-title><issue-title xml:lang="ru">ТОМ 18, №1 (2022)</issue-title><fpage>54</fpage><lpage>63</lpage><history><date date-type="received" iso-8601-date="2022-05-23"><day>23</day><month>05</month><year>2022</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2022, Galishnikova V.V., Elroba S.M., Dayoub N., Sakna A.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2022, Галишникова В.В., Эльроба Ш.М., Даюб Н., Сакна А.</copyright-statement><copyright-year>2022</copyright-year><copyright-holder xml:lang="en">Galishnikova V.V., Elroba S.M., Dayoub N., Sakna A.</copyright-holder><copyright-holder xml:lang="ru">Галишникова В.В., Эльроба Ш.М., Даюб Н., Сакна А.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">http://creativecommons.org/licenses/by/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://journals.rudn.ru/structural-mechanics/article/view/31050">https://journals.rudn.ru/structural-mechanics/article/view/31050</self-uri><abstract xml:lang="en"><p style="text-align: justify;">Microbiologically induced calcite precipitation, or calcium carbonate CaCO<sub>3</sub>, is used in remediating cracks and fissures in concrete. Since the microbial activity is pollution-free, natural, that process is extremely desired and may solve concrete cracking without sacrificing mechanical properties. The effects of different nutrient on the self-healing process are elucidated. Nutrients provide the required sources of energy for the bacterial growth and metabolic activities. A species of bacteria Bacillus sphaericus was added to the cement mix at a ratio of 0.6% of cement weight with three organic compounds for nutrients (calcium lactate, yeast extract and peptone) at 0.30% of cement weight. Effects on setting time, rate of water absorption, compressive strength and flexural strength were studied. It was found that bacteria nutrition acts as an accelerator for cement pastes for initial setting time mortar, while acts as a retarder of cement pastes for final setting time for all bacterial compared to control mortar. Finally, bacterial mortars with different types of nutrients showed an increase in compressive and flexural strengths with yeast extract showing the most promising enhancements, resulting in 26.5 and 60% increase in compressive and flexural strength respectively.</p></abstract><trans-abstract xml:lang="ru"><p style="text-align: justify;">Микробиологически индуцированное осаждение кальцита, или карбоната кальция CaCO<sub>3</sub>, используется для устранения трещин в бетоне. Микробная активность не загрязняет окружающую среду, поэтому данный процесс очень важен, а также он помогает решить проблему растрескивания бетона без ущерба для механических свойств. Выясняется влияние различных питательных веществ на процесс самовосстановления. Питательные вещества обеспечивают необходимые источники энергии для роста бактерий и метаболической деятельности. Вид бактерий Bacillus sphaericus был добавлен в цементную смесь в соотношении 0,6 % от массы цемента с тремя органическими соединениями для питательных веществ (лактат кальция, дрожжевой экстракт и пептон) в количестве 0,30 % от массы цемента. Изучено влияние на время схватывания, скорость водопоглощения, прочность на сжатие и прочность при изгибе. Обнаружено, что бактериальное питание действует как ускоритель цементных паст для начального времени схватывания раствора и одновременно как замедлитель цементных паст для окончательного времени схватывания для всех бактерий по сравнению с контрольным раствором. Наконец, бактериальные растворы с различными типами питательных веществ показали увеличение прочности при сжатии и изгибе, при этом дрожжевой экстракт продемонстрировал наиболее многообещающие результаты, что привело к увеличению прочности при сжатии и изгибе на 26,5 и 60 % соответственно.</p></trans-abstract><kwd-group xml:lang="en"><kwd>crack</kwd><kwd>organic compounds</kwd><kwd>water absorption</kwd><kwd>microbial calcium carbonate</kwd><kwd>self-healing</kwd><kwd>compressive strength</kwd><kwd>Bacillus sphaericus</kwd><kwd>Bacillus sphaericus</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>трещина</kwd><kwd>органические соединения</kwd><kwd>водопоглощение</kwd><kwd>микробный карбонат кальция</kwd><kwd>самовосстановление</kwd><kwd>прочность при сжатии</kwd></kwd-group><funding-group/></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Ganendra G., De Muynck W., Ho A., Charalampous Arvaniti E., Hosseinkhani B., Ramos J.A., Rahier H., Boon N. Formate oxidation-driven calcium carbonate precipitation by methylocystis parvus OBBP. Appl. Environ. Microbiol. 2014;80(15):4659-4667. http://doi.org/10.1128/AEM.01349-14</mixed-citation></ref><ref id="B2"><label>2.</label><mixed-citation>Zhang X., Jin Z., Li M., Qian C. Effects of carrier on the performance of bacteria-based self-healing concrete. Construction and Building Materials. 2021;305:124771. https://doi.org/10.1016/J.CONBUILDMAT.2021.124771</mixed-citation></ref><ref id="B3"><label>3.</label><mixed-citation>Vekariya M.S., Pitroda J. Bacterial concrete: new era for construction industry. Int. J. Eng. Trends Technol. 2013; 4(9):4128-4137. Available from: http://www.ijettjournal.org/volume-4/issue-9/IJETT-V4I9P181.pdf (acessed: 09.01.2022).</mixed-citation></ref><ref id="B4"><label>4.</label><mixed-citation>Seifan M., Samani A.K., Berenjian A. New insights into the role of pH and aeration in the bacterial production of calcium carbonate (CaCO3). Appl. Microbiol. Biotechnol. 2017;101(8):3131-3142. http://doi.org/10.1007/s00253-017-8109-8</mixed-citation></ref><ref id="B5"><label>5.</label><mixed-citation>Patil K., Waghere B., Salve R. Effect of bacterial calcite precipitation on compressive strength of mortar cubes. Int. J. Eng. Adv. Technol. 2013;2(3):486-491. Available from: http://www.ijeat.org/attachments/File/v2i3/C1186022313.pdf (acessed: 09.01.2022).</mixed-citation></ref><ref id="B6"><label>6.</label><mixed-citation>Zhang W., Zheng Q., Ashour A., Han B. Self-healing cement concrete composites for resilient infrastructures: a review. Composites Part B: Engineering. 2020;189:107892. https://doi.org/10.1016/J.COMPOSITESB.2020.107892</mixed-citation></ref><ref id="B7"><label>7.</label><mixed-citation>Qian C., Zheng T., Zhang X., Su Y. Application of microbial self-healing concrete: case study. Construction and Building Materials. 2021;290:123226. https://doi.org/10.1016/J.CONBUILDMAT.2021.123226</mixed-citation></ref><ref id="B8"><label>8.</label><mixed-citation>Mohammed H., Ortoneda-Pedrola M., Nakouti I., Bras A. Experimental characterisation of nonencapsulated bio-based concrete with self-healing capacity. Construction and Building Materials. 2020;256:119411. https://doi.org/10.1016/J.CONBUILDMAT.2020.119411</mixed-citation></ref><ref id="B9"><label>9.</label><mixed-citation>Roghanian N., Banthia N. Development of a sustainable coating and repair material to prevent bio-corrosion in concrete sewer and waste-water pipes. Cement and Concrete Composites. 2019;100:99-107. https://doi.org/10.1016/J.CEMCONCOMP.2019.03.026</mixed-citation></ref><ref id="B10"><label>10.</label><mixed-citation>Tian Z., Tang X., Xiu Z., Zhou H., Xue Z. The mechanical properties improvement of environmentally friendly fly ash-based geopolymer mortar using bio-mineralization. Journal of Cleaner Production. 2022;332:130020. https://doi.org/10.1016/J.JCLEPRO.2021.130020</mixed-citation></ref><ref id="B11"><label>11.</label><mixed-citation>Wang J., Ersan Y.C., Boon N., De Belie N. Application of microorganisms in concrete: a promising sustainable strategy to improve concrete durability. Appl. Microbiol. Biotechnol. 2016;100(7):2993-3007. http://doi.org/10.1007/s00253-016-7370-6</mixed-citation></ref><ref id="B12"><label>12.</label><mixed-citation>Nain N., Surabhi R., Yathish N.V., Krishnamurthy V., Deepa T., Tharannum S. Enhancement in strength parameters of concrete by application of Bacillus bacteria. Construction and Building Materials. 2019;202:904-908. https://doi.org/10.1016/J.CONBUILDMAT.2019.01.059</mixed-citation></ref><ref id="B13"><label>13.</label><mixed-citation>Anderson R.K.I., Jayaraman K., Voisard D., Marison I.W., Stockar U. Von Heat flux as an on-line indicator of metabolic activity in pilot scale bioreactor during the production of Bacillus thuringiensis var. galleriae-based biopesticides. Thermochimica Acta. 2002;386(2):127-138. https://doi.org/10.1016/S0040-6031(01)00709-2</mixed-citation></ref><ref id="B14"><label>14.</label><mixed-citation>Zhang L.V., Nehdi M.L., Suleiman A.R., Allaf M.M., Gan M., Marani A., Tuyan M. Crack self-healing in bio-green concrete. Composites Part B: Engineering. 2021;227:109397. https://doi.org/10.1016/J.COMPOSITESB.2021.109397</mixed-citation></ref><ref id="B15"><label>15.</label><mixed-citation>Restuccia L., Reggio A., Ferro G.A., Tulliani J.M. New self-healing techniques for cement-based materials. Procedia Structural Integrity. 2017;3:253-260. https://doi.org/10.1016/J.PROSTR.2017.04.016</mixed-citation></ref><ref id="B16"><label>16.</label><mixed-citation>Edvardsen C. Water permeability and autogenous healing of cracks in concrete. ACI Materials Journal. 1999;96(4): 448-454. https://doi.org/10.14359/645</mixed-citation></ref><ref id="B17"><label>17.</label><mixed-citation>Hearn N. Self-sealing, autogenous healing and continued hydration: What is the difference? Mater. Struct. Constr.1998;31(8):563-567. http://doi.org/10.1007/bf02481539</mixed-citation></ref><ref id="B18"><label>18.</label><mixed-citation>Achal V., Mukherjee A., Reddy M.S. Microbial concrete: way to enhance the durability of building structures. J. Mater. Civ. Eng.2011;23(6):730-734. http://doi.org/10.1061/(ASCE)MT.1943-5533.0000159</mixed-citation></ref><ref id="B19"><label>19.</label><mixed-citation>Jonkers H.M., Thijssen A., Muyzer G., Copuroglu O., Schlangen E. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecol. Eng. 2010;36(2):230-235. http://doi.org/10.1016/j.ecoleng.2008.12.036</mixed-citation></ref><ref id="B20"><label>20.</label><mixed-citation>Wang J.Y., De Belie N., Verstraete W. Diatomaceous earth as a protective vehicle for bacteria applied for self-healing concrete. J. Ind. Microbiol. Biotechnol. 2012;39(4):567-577. http://doi.org/10.1007/s10295-011-1037-1</mixed-citation></ref><ref id="B21"><label>21.</label><mixed-citation>Basaran Bundur Z., Kirisits M.J., Ferron R.D. Biomineralized cement-based materials: impact of inoculating vegetative bacterial cells on hydration and strength. Cem. Concr. Res. 2015;67:237-245. http://doi.org/10.1016/j.cemconres.2014.10.002</mixed-citation></ref><ref id="B22"><label>22.</label><mixed-citation>Lee H.X.D., Wong H.S., Buenfeld N.R. Self-sealing of cracks in concrete using superabsorbent polymers. Cement and Concrete Research. 2016;79:194-208. https://doi.org/10.1016/J.CEMCONRES.2015.09.008</mixed-citation></ref><ref id="B23"><label>23.</label><mixed-citation>Chahal N., Siddique R. Permeation properties of concrete made with fly ash and silica fume: influence of ureolytic bacteria. Construction and Building Materials. 2013;49:161-174. https://doi.org/10.1016/J.CONBUILDMAT.2013.08.023</mixed-citation></ref><ref id="B24"><label>24.</label><mixed-citation>Kumar Jogi P., Vara Lakshmi T.V.S. Self healing concrete based on different bacteria: a review. Materials Today: Proceedings. 2021;43:1246-1252. https://doi.org/10.1016/J.MATPR.2020.08.765</mixed-citation></ref><ref id="B25"><label>25.</label><mixed-citation>Naji Givi A., Abdul Rashid S., Aziz F.N.A., Salleh M.A.M. The effects of lime solution on the properties of SiO2 nanoparticles binary blended concrete. Composites Part B: Engineering. 2011;42(3):562-569. http://doi.org/10.1016/j.compositesb.2010.10.002</mixed-citation></ref><ref id="B26"><label>26.</label><mixed-citation>Jang I., Son D., Kim W., Park W., Yi C. Effects of spray-dried co-cultured bacteria on cement mortar. Constr. Build. Mater. 2020;243:118206. http://doi.org/10.1016/j.conbuildmat.2020.118206</mixed-citation></ref><ref id="B27"><label>27.</label><mixed-citation>Tayebani B., Mostofinejad D. Self-healing bacterial mortar with improved chloride permeability and electrical resistance. Constr. Build. Mater. 2019;208:75-86. http://doi.org/10.1016/j.conbuildmat.2019.02.172</mixed-citation></ref></ref-list></back></article>
