RUDN Journal of MedicineRUDN Journal of Medicine2313-02452313-0261Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)3239Research ArticleCombined toxicity of Na 2EDTA and D 2OBurdeinayaT Nt_burdeynaya@yahoo.comZrelovO YDepartment of Pharmaceutical and Toxicological Chemistryzrelovoleg@rambler.ruMaksimovaT VDepartment of Pharmaceutical and Toxicological Chemistrymaximtat@mail.ruMorozovaM ADepartment of Pharmaceutical and Toxicological Chemistrygor-mariya@yandex.ruMteiG IDepartment of Pharmaceutical and Toxicological Chemistrygastoi@yahoo.comUspenskayaE VDepartment of Pharmaceutical and Toxicological Chemistryuspenskaya75@mail.ruPletenevaT VDepartment of Pharmaceutical and Toxicological Chemistrytvplet@mail.ruSyroeshkinA Vlivmatter@mail.ruZAO “Legkaya voda”Peoples’ Friendship University of RussiaInstitute of Applied Geophysics1502201325907092016Copyright © 2013,2013Physicochemical and biopharmaceutical analysis of excipients doesn’t allow to characterize them as inert components of drug products. Excipients are known to exhibit their own biological activity as well as influence the activity of API and other components of the formulation (antioxidants, stabilizers, fillers, etc.). Combined biological activity of stabilizer/antioxidant — disodium ethylenediaminetetraacetate (Na 2EDTA) — and water with different ratios of hydrogen isotopes (D / H) was studied on biological models of Spirotox. It was found that with the growth of deuterium/protium ratio in water the toxicity of Na 2EDTA increased.Spirotoxexcipientsbiopharmaceutical analysisheavy waterSpirotoxвспомогательные веществабиофармацевтический анализтяжелая вода