RUDN Journal of MedicineRUDN Journal of MedicineВестник Российского университета дружбы народов. Серия: Медицина2313-02452313-0261Peoples’ Friendship University of Russia named after Patrice Lumumba (RUDN University)1843710.22363/2313-0245-2018-22-1-43-49IMMUNOLOGY. INFECTIOUS PATHOLOGYИММУНОЛОГИЯ. ИНФЕКЦИОННАЯ ПАТОЛОГИЯResearch ArticleProteins and microRNAs involved in papillomavirus infectionБелки и микроРНК, участвующие в папилломавирусной инфекцииKlimovE AКлимовЕвгений Александрович

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доктор биологических наук, ведущий научный сотрудник, кафедра генетики биологического факультета МГУ имени М.В. Ломоносова, заместитель генерального директора ООО «Университетская диагностическая лаборатория», заместитель директора по науке ФГБНУ «Центр экспериментальной эмбриологии и репродуктивных биотехнологий».

klimov@mail.bio.msu.ruSobolevV VСоболевВ Вklimov@mail.bio.msu.ruSolov’evA MСоловьевА Мklimov@mail.bio.msu.ruPerlamutrovYu NПерламутровЮ Нklimov@mail.bio.msu.ruKorsunskayaI MКорсунскаяИ Мklimov@mail.bio.msu.ruLomonosov Moscow State UniversityМосковский государственный университет им. М.В. ЛомоносоваUniversity Diagnostic Laboratory, LLCООО Университетская диагностическая лабораторияCenter of Experimental Embryology and Reproductive BiotechnologiesФГБНУ «Центр экспериментальной эмбриологии и репродуктивных биотехнологий»I.I. Mechnikov Research Institute for Vaccines and SeraФГБУ «Научно-исследовательский институт вакцин и сывороток им. И.И. Мечникова» РАНCentre of Theoretical Problems of Physico-Chemical PharmacologyФГБУН «Центр теоретических проблем физико-химической фармакологии Российской академии наук»A.I. Yevdokimov Moscow State University of Medicine and DentistryФГБОУ ВО «Московский государственный медико-стоматологический университет имени А.И. Евдокимова»15122018221VOL 22, NO1 (2018)ТОМ 22, №1 (2018)434901052018Copyright ©; 2018, Klimov E.A., Sobolev V.V., Solov’ev A.M., Perlamutrov Y.N., Korsunskaya I.M.Copyright ©; 2018, Климов Е.А., Соболев В.В., Соловьев А.М., Перламутров Ю.Н., Корсунская И.М.2018Klimov E.A., Sobolev V.V., Solov’ev A.M., Perlamutrov Y.N., Korsunskaya I.M.Климов Е.А., Соболев В.В., Соловьев А.М., Перламутров Ю.Н., Корсунская И.М.http://creativecommons.org/licenses/by/4.0https://journals.rudn.ru/medicine/article/view/18437

The review is devoted to the analysis of proteins, protein complexes and microRNAs involved in papillomavirus infection. The papilloma viruses belong to the family of DNA-containing papovaviruses (Papovaviridae) and infect the basal layers of the epithelium. Human papillomaviruses (HPV) have an oncogenic potential, which varies significantly. The ring genome HPV encodes 6 early proteins and 2 later proteins (envelope proteins), and uses the host cell proteins for its own replication. For infection the membrane proteins that bind to the host cell membrane are especially important. In this paper we analyzed the arrays of literature data on proteins and microRNAs involved in binding, internalization, transport of HPV virions, and virus multiplication. The analysis of literature data was carried out using the program PathwayStudio9 ® and the database ResNet13 ® of Elsivier (USA), as well as online services. Total was revealed 69 infection-related proteins and protein complexes and 3 microRNAs (72 in total). As a result of the subsequent detailed analysis of the literature, 17 proteins and microRNAs involved in the following processes were selected: penetration of virus particles into the cell (7), transport of the virus in the cytoplasm of the host cell (5), transfer of the virus DNA into the core of the host cell (1) and regulation of gene transcription HPV (4). These proteins and microRNAs are potential targets for drugs that can prevent and suppress HPV infection.

Обзор посвящен анализу белков, белковых комплексов и микроРНК, участвующих в папилломовирусной инфекции. Вирусы папилломы относятся к семейству ДНК-содержащих паповавирусов (Papovaviridae) и инфицируют базальные слои эпителия. Вирусы папилломы человека (ВПЧ) имеют онкогенный потенциал, который существенно варьирует. Кольцевой геном ВПЧ кодирует 6 ранних белков и 2 поздних (белки оболочки), а для собственной репликации использует белки клетки хозяина. Для заражения особо важны белки оболочки, связывающиеся с мембраной клетки хозяина. Учитывая, что в большинстве процессов папилломавирусной инфекции участвуют белки клетки-хозяина, представляется интересным рассмотреть эти белки и их роль в развитии инфекции. В данной работе мы провели анализ массивов литературных данных о белках и микроРНК, участвующих в связывании, интернализации, транспорте вирионов ВПЧ, а также размножении вируса. Анализ литературных данных проведен с использованием программы PathwayStudio9 ® и реферативной базы данных ResNet13 ® компании Elsivier (США), а также онлайн сервисов. Всего было выявлено 69 связанных с инфекцией белков и белковых комплексов и 3 микроРНК (всего 72 объекта). В результате последующего детального анализа литературы отобрано 17 белков и микроРНК, участвующих в следующих процессах: проникновение вирусных частиц в клетку (7 белков), транспорт вируса в цитоплазме клетки хозяина (5 белков), перенос ДНК вируса в ядро клетки хозяина (1 белок) и регуляция транскрипции генов ВПЧ (2 белка и 2 микроРНК). Эти белки и микроРНК являются потенциальными мишенями для препаратов, способных предотвращать и подавлять инфекцию ВПЧ.

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