Selection of the cell culture to accumulate in vitro the sacbrood virus of bees

Abstract


Bees, like other living organisms are exposed to various pathogenic microorganisms, such as bacteria, fungi, protozoans, parasites and viruses. Viruses are of particular threat to beekeeping. Some diseases caused by viruses that lead to the collapse of the bee colonies, which leads to loss of a huge number of bees and it causes a recession in the industry. One of the major bees diseases caused by viruses is a sacculated brood. This pathology causes significant damage to beekeeping, because sick families without assistance does not give the commodity honey and not able to provide themselves food, then farms lose the opportunity to sell queens and bee packages. Problem of prompt diagnosis is inaccessibility of bees culturing cells method in vitro virus, which is why understanding of the biological mechanisms underlying viral diseases is limited. These difficulties in the virologists works leads to late diagnosis of disease and as a result, there are large losses in farms. Currently, there is very little information about the cultivation of viruses bees on heterogeneous cell cultures, which give after passage of the pathogen any degenerative changes. However, in last few years there is an active debate on the cultivation of bees viruses in cell cultures. It is known that the highest sensitivity to the saccular brood virus are explants of ovarian nymphs queens bees, chick fibroblast culture, bee fibroblasts tissue of the pectoral muscles of adult bees, culture of the tissue reproductive organs, muscle and egg bee primary trypsinized culture of monkey kidney, primary cell culture, obtained from the larvae of worker bees, culture PC-15, IBRS-2. The major problem of cultivation of bees viruses - a small amount of data on heterologous type of sensitive cell cultures. However, research in this area continues, and scientists around the world are trying to solve a serious problem of cultivation of bee viruses.


A G Kalinin

Principal contact for editorial correspondence.
kalinin.andrew2010@yandex.ru
Peoples’ Friendship University of Russia (RUDN University) Miklukho-Maklay str., 8/9, Moscow, Russia, 117198

FGBNU All-Russian Research Institute of Experimental Veterinary Medicine. YR Kovalenko Ryazan prosp., 24-1, Moscow, Russia, 109428

T V Galnbek

admin@viev.ru
FGBNU All-Russian Research Institute of Experimental Veterinary Medicine. YR Kovalenko Ryazan prosp., 24-1, Moscow, Russia, 109428

E V Kulikov

kulikov_ev@rudn.university
Peoples’ Friendship University of Russia (RUDN University) Miklukho-Maklay str., 8/9, Moscow, Russia, 117198

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